Fuglesang J E, Bergan T, Bielecki T, Naterstad A, Namork E
Infection. 1981;9(6):290-5. doi: 10.1007/BF01640994.
Mecillinam, ampicillin and combinations of the two were tested against Enterobacteriaceae by traditional agar dilution and under in vitro conditions simulating in vivo pharmacokinetics. Synergy was demonstrated by agar dilution in 30% of the strains. At simulated in vivo conditions, synergy between ampicillin and mecillinam was detected in a high osmolality medium, but not when the osmolality of the medium was low. The bactericidal effect was detected with mecillinam after one hour, while that of ampicillin or the combination was detected after 15 min. Changed bacterial morphology was observed by electron microscopy. Mecillinam produced ovoid spherical cell structures whereas combinations of the two drugs produced larger bacterial spheres.
采用传统琼脂稀释法，并在模拟体内药代动力学的体外条件下，对美西林、氨苄西林以及二者的组合进行了针对肠杆菌科细菌的测试。通过琼脂稀释法在30%的菌株中证实了协同作用。在模拟体内条件下，在高渗培养基中检测到氨苄西林与美西林之间有协同作用，但在培养基渗透压较低时则未检测到。一小时后检测到美西林的杀菌作用，而氨苄西林或二者组合的杀菌作用在15分钟后检测到。通过电子显微镜观察到细菌形态发生了变化。美西林产生了卵形球状细胞结构，而两种药物的组合产生了更大的细菌球体。
