Schat K A, Shek W R, Calnek B W, Abplanalp H
Int J Cancer. 1982 Feb 15;29(2):187-94. doi: 10.1002/ijc.2910290213.
Cell-mediated cytotoxicity (CMC) of lymphocytes obtained from chickens infected with Marek's disease (MD) virus against allogeneic MD lymphoblastoid cell lines has been reported by several research groups. Recently, we established a number of cell lines from MD tumors obtained from highly inbred chickens and characterized for major and minor histocompatibility antigens. Allogeneic versus syngeneic CMC was studied using those cell lines and lymphocytes obtained from chickens 6-8 days post infection with SB-1, a non-oncogenic MD virus. Allogeneic cytotoxicity could be easily demonstrated, while syngeneic cytotoxicity was a rare event. However, increase of the CMC assay period from 4 to 8 h did enhance syngeneic cytotoxicity. Cold inhibition assays demonstrated that the allogeneic cytotoxicity was directed against alloantigens present on spleen lymphocytes sharing the same major histocompatibility antigens as the target cells. Cytotoxicity was not influenced by the sex of either target or effector cells or by the level of virus infectivity of the effector cells.
几个研究小组报告了从感染马立克氏病（MD）病毒的鸡中获得的淋巴细胞对同种异体MD淋巴母细胞系的细胞介导细胞毒性（CMC）。最近，我们从高度近交鸡的MD肿瘤中建立了许多细胞系，并对主要和次要组织相容性抗原进行了表征。使用这些细胞系和从感染非致癌性MD病毒SB-1后6-8天的鸡中获得的淋巴细胞研究了同种异体与同基因CMC。同种异体细胞毒性很容易证明，而同基因细胞毒性则很少见。然而，将CMC检测时间从4小时延长到8小时确实增强了同基因细胞毒性。冷抑制试验表明，同种异体细胞毒性针对的是与靶细胞共享相同主要组织相容性抗原的脾淋巴细胞上存在的同种抗原。细胞毒性不受靶细胞或效应细胞的性别或效应细胞的病毒感染水平的影响。
