小鼠可移植性产病毒皮脂腺腺瘤中的组织连接。II. 结合制霉菌素的冷冻断裂研究。
Tissue connections in a transplantable virus-producing sebaceous adenoma of the mouse. II. A freeze-fracture study in conjunction with filipin.
作者信息
Robenek H, Schöpper C, Fasske E, Fetting R, Themann H
出版信息
J Cancer Res Clin Oncol. 1982;102(3):215-26. doi: 10.1007/BF00411341.
The distribution, size, and configuration of intercellular junctions in the sebaceous tumor of mice were examined using the freeze-fracture technique. Three types of junctions were observed: desmosomes, gap, and tight junctions. Tight junctions in general consisted of short linear unbranched fibrils, and macular or complex tight junctional patterns were present only occasionally. Gap junctions ranged from small sports of 0.9 x 10(-3) micrometer2 to areas of approximately 0.46 micrometers2. Desmosomes were the most frequent junctional specializations, and it is concluded that they are at least partially responsible for this tumor not being metastatic. Filipin in conjunction with freeze-fracture showed filipin-sterol complexes on the plasma membrane, the nuclear envelope, and the membranes of the endoplasmic reticulum and mitochondria. The intercellular junctions were devoid of these complexes indicating that these regions are low in cholesterol.
利用冷冻断裂技术检查了小鼠皮脂腺肿瘤中细胞间连接的分布、大小和形态。观察到三种类型的连接：桥粒、缝隙连接和紧密连接。紧密连接一般由短的线性无分支纤维组成，仅偶尔出现斑状或复杂的紧密连接模式。缝隙连接的范围从0.9×10⁻³平方微米的小区域到约0.46平方微米的区域。桥粒是最常见的连接特化结构，得出的结论是它们至少部分地导致了这种肿瘤不发生转移。 Filipin与冷冻断裂技术结合显示，在质膜、核膜、内质网和线粒体膜上有Filipin - 固醇复合物。细胞间连接没有这些复合物，表明这些区域胆固醇含量低。
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