肝微粒体脂质过氧化过程中自由基的自旋捕获
Spin trapping of free radicals during hepatic microsomal lipid peroxidation.
作者信息
Rosen G M, Rauckman E J
出版信息
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7346-9. doi: 10.1073/pnas.78.12.7346.
Abstract
We have used spin-trapping techniques to identify radical species formed during the NADPH-stimulated peroxidation of rat hepatic microsomes. Using 5,5-dimethyl-1-pyrroline-1-oxide, we have confirmed the presence of substantial quantities of superoxide but found evidence for the formation of only small quantities of hydroxyl radical. Use of the spin traps N-tert-butyl-alpha-4-pyridylnitrone-1-oxide and 2-methyl-2-nitroso-1-propanol have allowed us to determine that lipid peroxyl radicals are the predominant "lipid-type" radical found in peroxidizing microsomes under aerobic conditions. These data suggest that microsomal lipid dienyl radicals react with molecular oxygen at near diffusion-controlled rates.
摘要
我们已使用自旋捕获技术来鉴定在NADPH刺激的大鼠肝微粒体过氧化过程中形成的自由基种类。使用5,5-二甲基-1-吡咯啉-1-氧化物，我们已证实存在大量超氧化物，但仅发现少量羟基自由基形成的证据。使用自旋捕获剂N-叔丁基-α-4-吡啶基硝酮-1-氧化物和2-甲基-2-亚硝基-1-丙醇，使我们能够确定脂质过氧自由基是在有氧条件下过氧化微粒体中发现的主要“脂质型”自由基。这些数据表明微粒体脂质二烯基自由基以接近扩散控制的速率与分子氧反应。
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