Souhami R L, Babbage J, Callard R E
Clin Exp Immunol. 1981 Oct;46(1):98-105.
Peripheral blood mononuclear cells from normal donors were stimulated in vitro by varicella zoster (VZ) antigen and antibody measured by an ELISA technique. Antibody was produced in 14 of 19 individuals. This antibody was specific for VZ with no binding to herpes simplex virus (HSV), even when the mononuclear cells were taken from individuals who had circulating antibody to VZ and HSV. The failure of antibody production in mononuclear cell cultures from some normals, all of whom had serum antibody, was analysed using reciprocal combinations of E+ and E- cells from producers and non-producers. E+ cells from producers could help E- producer cells make antibody, but E+ cells from non-producers could not help E- cells from non-producers to do so. This result suggests a deficiency in circulating E- cells in non-producers. Four of four patients with untreated Hodgkin's disease were non-producers using blood mononuclear cell cultures, but their spleen cells produced antibody readily. This suggests that failure of in vitro antibody production in immune individuals may be due to sequestration of responsive B cells in spleen and other sites. This technique allows further study of the mechanisms of immunity to VZ in normal and immune-compromised individuals.
用ELISA技术检测水痘带状疱疹（VZ）抗原刺激正常供体的外周血单个核细胞后产生的抗体。19名个体中有14名产生了抗体。该抗体对VZ具有特异性，不与单纯疱疹病毒（HSV）结合，即使单核细胞取自对VZ和HSV有循环抗体的个体。使用来自产生者和非产生者的E+和E-细胞的相互组合，分析了一些所有血清中都有抗体的正常个体的单核细胞培养物中抗体产生失败的情况。来自产生者的E+细胞可以帮助E-产生者细胞产生抗体，但来自非产生者的E+细胞不能帮助来自非产生者的E-细胞产生抗体。这一结果表明非产生者中循环E-细胞存在缺陷。4例未经治疗的霍奇金病患者的血液单核细胞培养物均为非产生者，但其脾细胞很容易产生抗体。这表明免疫个体体外抗体产生失败可能是由于反应性B细胞在脾脏和其他部位被隔离。该技术有助于进一步研究正常个体和免疫功能低下个体对VZ的免疫机制。
