Lachmann P J, Macanovic M, Harkiss G D, Oldroyd R G, Habicht J
Clin Exp Immunol. 1981 Nov;46(2):250-8.
A technique is described which allows the antibodies of circulating immune complexes to be isolated as their F(ab')2 fragments. The method is based on the precipitation of the complexes by the sequential addition of conglutinin and anti-conglutinin, and the subsequent digestion of these precipitates by pepsin. Using this technique it has been possible to show antibodies to Epstein-Barr (EB) virus antigens in the immune complexes of patients with Burkitt's lymphoma and to microbial antigens in two patients with nephritis. By substituting DNAase for pepsin it has also been possible to show antibodies to DNA-containing nuclear antigens in the serum of patients with systemic lupus erythematosus.
本文描述了一种技术，该技术可将循环免疫复合物中的抗体分离为其F(ab')2片段。该方法基于通过依次添加胶固素和抗胶固素使复合物沉淀，以及随后用胃蛋白酶消化这些沉淀物。使用该技术已能够在伯基特淋巴瘤患者的免疫复合物中检测到针对爱泼斯坦-巴尔（EB）病毒抗原的抗体，以及在两名肾炎患者的免疫复合物中检测到针对微生物抗原的抗体。通过用脱氧核糖核酸酶替代胃蛋白酶，还能够在系统性红斑狼疮患者的血清中检测到针对含DNA核抗原的抗体。
