Wamsley J K, Zarbin M A, Young W S, Kuhar M J
Neuroscience. 1982 Mar;7(3):595-613. doi: 10.1016/0306-4522(82)90066-5.
By employing both in vivo and in vitro labeling techniques, opiate receptors were labeled with tritiated diprenorphine in the monkey brain and localized by light microscopic autoradiography. Both methods of labeling gave similar results, allowing a description of discrete areas having opiate binding sites. High concentrations of opiate receptors were found in the substantia gelatinosa of the spinal cord, nucleus tractus solitarius, area postrema, lateral parabrachial nucleus, substantia grisea centralis, several nuclei of the thalamus and hypothalamus, substantia innominata and in the amygdala. In the monkey pituitary, receptors were found in the neurohypophysis. These results correlate well with those found in autoradiographic studies of the rat brain although there are a few notable differences. Many of the opiate receptor distributions can be correlated with anatomical loci of brain functions known to be influenced by administration of opiate compounds.
通过运用体内和体外标记技术，用氚化二丙诺啡对猴脑内的阿片受体进行标记，并通过光学显微镜放射自显影法进行定位。两种标记方法得出了相似的结果，从而能够描述具有阿片结合位点的离散区域。在脊髓胶状质、孤束核、最后区、外侧臂旁核、中央灰质、丘脑和下丘脑的几个核团、无名质以及杏仁核中发现了高浓度的阿片受体。在猴垂体中，神经垂体发现有受体。这些结果与在大鼠脑放射自显影研究中发现的结果密切相关，尽管存在一些显著差异。许多阿片受体分布可与已知受阿片类化合物给药影响的脑功能解剖位点相关联。
