Ducatelle R, Coussement W, Debouck P, Hoorens J
Vet Pathol. 1982 Jan;19(1):57-66. doi: 10.1177/030098588201900109.
Sixteen cesarean-derived, colostrum-deprived piglets were infected oronasally with CV777 coronavirus on the second or third day of life. Two uninfected piglets were controls. After an incubation period of 22 hours to 36 hours, all principals showed severe diarrhea. The piglets were killed at different time intervals. Viral particles were found in the jejunal villous epithelial cells from 18 hours after infection until four days after the beginning of diarrhea. In the colonic epithelial cells, viral particles and degenerative lesions were found only in the piglet killed 36 hours after onset of diarrhea. Degenerative lesions in the enterocytes began at 18 hours after infection and were most pronounced in the jejunum at the onset of clinical signs. From 24 hours on after the onset of clinical signs, three cell types were found: degenerated virus-containing enterocytes; cuboidal cells; and columnar, highly vacuolated cells containing lipid droplets.
16只剖腹产、未摄取初乳的仔猪在出生后第二天或第三天经口感染CV777冠状病毒。2只未感染的仔猪作为对照。在22小时至36小时的潜伏期后，所有主要受试仔猪均出现严重腹泻。在不同时间间隔处死仔猪。从感染后18小时直至腹泻开始后4天，在空肠绒毛上皮细胞中均发现病毒颗粒。在结肠上皮细胞中，仅在腹泻开始后36小时处死的仔猪中发现病毒颗粒和退行性病变。肠上皮细胞的退行性病变在感染后18小时开始，在临床症状出现时空肠中最为明显。临床症状出现后24小时起，发现三种细胞类型：退化的含病毒肠上皮细胞；立方形细胞；以及含有脂滴的柱状、高度空泡化细胞。
