Carman S, Povey C
Can J Comp Med. 1982 Jan;46(1):33-8.
Withholding food from dogs for 24 hours prior to, and for 48 hours following oral challenge with a gut mucosal homogenate of canine parvovirus-2, was a successful means of reproducing gastroenteric signs of canine parvovirus-2 infection. Twenty-one of 24 dogs, which had previously received various vaccine preparations of mink enteritis virus or were unvaccinated, and which were starved at challenge, developed soft or liquid feces with large or without large clots of mucus. Altered feces were most frequent on postexposure day 11. Seven dogs passed frank blood in their stools on one or more occasions and seven dogs vomited sporadically. Pyrexia was noted in 71.6% of the dogs on postexposure day 6 and lymphopenia was detected on postexposure day 5 or 6 in 50% of the dogs monitored. In contrast, four dogs not starved at the time of challenge remained free of gastrointestinal signs apart from one dog which passed a soft stool with scant mucus on one day, postexposure day 6. Also four dogs vaccinated with a killed canine parvovirus-2 vaccine preparation and subsequently starved at the time of challenge, remained clinically healthy. Apart from these last mentioned four dogs, all others shed canine parvovirus-2 in their feces following challenge.
在用犬细小病毒2型肠道黏膜匀浆进行口服攻毒之前24小时以及攻毒之后48小时不给犬喂食，是再现犬细小病毒2型感染胃肠症状的一种成功方法。24只犬中，有21只之前接种过各种水貂肠炎病毒疫苗制剂或未接种过疫苗，且在攻毒时处于饥饿状态，它们出现了软便或稀便，伴有或不伴有大量黏液凝块。在接触病毒后第11天，粪便改变最为常见。7只犬在一个或多个时间点粪便中出现明显血液，7只犬偶尔呕吐。在接触病毒后第6天，71.6%的犬出现发热，在接触病毒后第5天或第6天，接受监测的犬中有50%检测到淋巴细胞减少。相比之下，4只在攻毒时未饥饿的犬除了1只在接触病毒后第6天排出一次带有少量黏液的软便外，没有出现胃肠道症状。另外4只接种了灭活犬细小病毒2型疫苗制剂且随后在攻毒时处于饥饿状态的犬，临床上保持健康。除了最后提到的这4只犬外，所有其他犬在攻毒后粪便中都排出了犬细小病毒2型。
