Penn C W, Sen D, Veale D R, Parsons N J, Smith H
J Gen Microbiol. 1976 Nov;97(1):35-43. doi: 10.1099/00221287-97-1-35.
Gonococci (strain BS3) passaged three times and harvested directly from plastic chambers implanted subcutaneously in guinea pigs were compared with the parent strain (BS) grown in vitro. The strain grown in vivo produced smaller colonies than that grown in vitro and when examined directly in chamber fluid was sometimes not pilated. It was more resistant to the bactericidal action of human serum and more infective for guinea-pig chambers. In gel diffusion, extracts of the organisms adapted in vivo and cultured once on agar appeared to contain one or two antigens that were different from those in extracts of the in vitro grown organisms; and on polyacrylamide gels, electrophoresis of similar extracts showed one or more protein components for strain BS3 which were not seen for strain BS. Gonococci grown in guinea-pig subcutaneous chambers appear to be suitable for studies on the determinants of gonoccal pathogenicity.
将在豚鼠皮下植入的塑料小室中传代三次并直接收获的淋球菌（菌株BS3）与在体外培养的亲代菌株（BS）进行比较。体内生长的菌株产生的菌落比体外生长的菌株小，直接在小室液体中检查时有时没有菌毛。它对人血清的杀菌作用更具抗性，对豚鼠小室的感染性更强。在凝胶扩散试验中，体内适应并在琼脂上培养一次的菌株提取物似乎含有一两种与体外生长菌株提取物不同的抗原；在聚丙烯酰胺凝胶上，类似提取物的电泳显示菌株BS3有一个或多个蛋白质成分，而菌株BS没有。在豚鼠皮下小室中生长的淋球菌似乎适合用于研究淋球菌致病性的决定因素。
