Albritton W L, Brunton J L, Slaney L, MacLean I
Antimicrob Agents Chemother. 1982 Jan;21(1):159-65. doi: 10.1128/AAC.21.1.159.
Clinical isolates of Haemophilus ducreyi from patients with chancroid were shown to have one or more 4.9- to 7.0-megadalton non-self-transferable plasmids and to have in vitro resistance to sulfonamides. Transformation of Escherichia coli to sulfonamide resistance was associated with the acquisition of a 4.9-megadalton plasmid, which did not confer linked resistance to streptomycin. The guanine-plus-cytosine content of this plasmid was found to be 57%. Filter-blot hybridization and restriction endonuclease digestion studies suggested a relationship of this plasmid to RSF1010. Electron microscope heteroduplex analysis confirmed this relationship. The identification in H. ducreyi of a plasmid closely related to plasmids found in enteric species, rather than transposition of a resistance determinant to an indigenous plasmid, suggests that further dissemination of the enteric plasmid pool to this genus is possible since plasmid transfer between certain Haemophilus species is readily demonstrated.
从软下疳患者分离出的杜克雷嗜血杆菌临床菌株显示有一个或多个4.9至7.0兆道尔顿的非自我转移性质粒，并且对磺胺类药物有体外抗性。将大肠杆菌转化为磺胺抗性与获得一个4.9兆道尔顿的质粒有关，该质粒并未赋予对链霉素的连锁抗性。发现该质粒的鸟嘌呤加胞嘧啶含量为57%。滤膜杂交和限制性内切酶消化研究表明该质粒与RSF1010有关。电子显微镜异源双链分析证实了这种关系。在杜克雷嗜血杆菌中鉴定出与肠道菌中发现的质粒密切相关的质粒，而不是抗性决定簇转座到本地质粒，这表明肠道质粒库有可能进一步传播到该属，因为在某些嗜血杆菌属物种之间很容易证明质粒转移。
