Ansari K A, Hendrickson H, Rand A
J Neuropathol Exp Neurol. 1976 Nov-Dec;35(6):606-12. doi: 10.1097/00005072-197611000-00002.
Histologically confirmed normal pieces of human white matter removed during surgical approach to underlying pathology were studied by acrylamide disc gel electrophoresis and electron microscopy. A basic electrophoretic pattern of the white matter homogenates from three separate patients is described. Aliquots of white matter from two of these patients were incubated at 4 degrees C and 23 degrees C for intervals up to 18 hours, then homogenized and electrophoresed to detect any degradative changes in the basic protein band. Results of these studies indicated that the basic protein band of freshly obtained normal human white matter was unaffected by incubation at 23 degrees C for as long as 18 hours. Electron microscopic examination of white matter that was incubated for 2 hours at room temperature prior to fixation, showed sporadic areas of lamellar separation, a finding similar to but not as extensive as that described earlier in white matter obtained at autopsy that was performed 8 hours post-mortem. These findings 1) confirm earlier observations made on autopsy material, 2) are compatible with location of basic protein along the cytoplasmic surface of myelin lamellae, and 3) further emphasize the remarkable resistance of basic protein in situ to autolytic degradation.
通过丙烯酰胺圆盘凝胶电泳和电子显微镜对手术切除的组织学确诊的正常人类白质样本进行研究，这些白质样本是在处理潜在病理状况的手术过程中获取的。描述了来自三名不同患者的白质匀浆的基本电泳图谱。取其中两名患者的白质等分试样，分别在4℃和23℃下孵育长达18小时，然后匀浆并进行电泳，以检测碱性蛋白带中的任何降解变化。这些研究结果表明，新鲜获取的正常人白质的碱性蛋白带在23℃下孵育长达18小时不受影响。对在固定前于室温下孵育2小时的白质进行电子显微镜检查，发现有散在的板层分离区域，这一发现与之前在死后8小时进行尸检获得的白质中描述的情况相似，但程度没那么严重。这些发现：1）证实了之前对尸检材料的观察结果；2）与碱性蛋白沿髓鞘板层细胞质表面的定位相符；3）进一步强调了原位碱性蛋白对自溶降解具有显著抗性。
