前列腺素A2对大鼠脑突触质膜Na+-K+-ATP酶活性的体外抑制作用
Inhibitory effect of prostaglandin A2 on Na+-K+-ATPase activity in synaptic plasma membrane of rat brain in vitro.
作者信息
Shibata Y, Ohzeki H, Sato M, Suzuki Y, Takiguchi H
出版信息
Int J Biochem. 1982;14(5):347-50. doi: 10.1016/0020-711x(82)90016-7.
PMID:6282665
Abstract
- The Na+-K+-ATPase activity in the synaptic plasma membrane of rat brain was inhibited by PGA2 in vitro. 2. PGA2 concentration required for 50% inhibition was 8.91 x 10(-5) M. 3. Inhibition mode of PGA2 for both ATP and Mg2+ was uncompetitive type. 4. PGA2 was competitive and noncompetitive with Na+ and K+, respectively. 5. The Ki values for PGA2 inhibition for ATP. Na+, K+ and Mg2+ were 6.0 x 10(-5), 2.7 x 10(-5), 10.8 x 10(-5) and 0.6 x 10(-5) M, respectively.
摘要
- 体外实验中，PGA2可抑制大鼠大脑突触质膜中的钠钾ATP酶活性。2. 半数抑制所需的PGA2浓度为8.91×10⁻⁵ M。3. PGA2对ATP和Mg²⁺的抑制模式均为非竞争性。4. PGA2对Na⁺具有竞争性，对K⁺具有非竞争性。5. PGA2抑制ATP、Na⁺、K⁺和Mg²⁺的Ki值分别为6.0×10⁻⁵、2.7×10⁻⁵、10.8×10⁻⁵和0.6×10⁻⁵ M。
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