Curiale M S, Levy S B
J Bacteriol. 1982 Jul;151(1):209-15. doi: 10.1128/jb.151.1.209-215.1982.
The structural and regulatory tetracycline resistance genes of transposon Tn10 are located on a 2,700-base pair HpaI fragment. We have used eight tetracycline-sensitive mutations in the 2,700-base pair fragment, cloned into two compatible plasmids, to demonstrate that two complementation groups are required for tetracycline resistance. By genetic recombination with plasmids containing the regulatory or structural regions for resistance, we have determined that both complementation groups reside within the structural region. The complementation groups, designated tetA and tetB, are proximal and distal, respectively, to the promoter for the tetracycline resistance structural region. The tetB mutations are in the portion of the structural region that is known to encode the 36,000-molecular-weight, inner-membrane TET protein. The levels of tetracycline resistance expressed during complementation suggest a complex interaction between the products of the tetA and tetB loci.
转座子Tn10的结构和调控四环素抗性基因位于一个2700碱基对的HpaI片段上。我们利用该2700碱基对片段中的八个四环素敏感突变体，将其克隆到两个相容的质粒中，以证明四环素抗性需要两个互补群。通过与含有抗性调控或结构区域的质粒进行基因重组，我们确定两个互补群均位于结构区域内。分别命名为tetA和tetB的互补群，分别位于四环素抗性结构区域启动子的近端和远端。tetB突变位于结构区域中已知编码36000分子量内膜TET蛋白的部分。互补过程中表达的四环素抗性水平表明tetA和tetB基因座产物之间存在复杂的相互作用。
