一个MATE家族多药外排转运蛋白将氟喹诺酮类药物泵出多形拟杆菌。
A MATE family multidrug efflux transporter pumps out fluoroquinolones in Bacteroides thetaiotaomicron.
作者信息
Miyamae S, Ueda O, Yoshimura F, Hwang J, Tanaka Y, Nikaido H
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3206, USA.
出版信息
Antimicrob Agents Chemother. 2001 Dec;45(12):3341-6. doi: 10.1128/AAC.45.12.3341-3346.2001.
Abstract
We cloned a gene, bexA, that codes for a multidrug efflux transporter from the chromosomal DNA of Bacteroides thetaiotaomicron ATCC 29741 by using an Escherichia coli DeltaacrAB DeltaacrEF mutant as a host. Although the initial recombinant construct contained other open reading frames, the presence of bexA alone was sufficient to confer to the E. coli host elevated levels of resistance to norfloxacin, ciprofloxacin, and ethidium bromide. Disruption of bexA in B. thetaiotaomicron made the strain more susceptible to norfloxacin, ciprofloxacin, and ethidium bromide, showing that this gene is expressed in this organism and functions as a multidrug efflux pump. The deduced BexA protein sequence was homologous to the protein sequence of Vibrio parahaemolyticus NorM, a multidrug efflux transporter, and thus, BexA belongs to the multidrug and toxic compound extrusion (MATE) family.
摘要
我们通过使用大肠杆菌ΔacrAB ΔacrEF突变体作为宿主，从嗜热栖热放线菌ATCC 29741的染色体DNA中克隆了一个编码多药外排转运蛋白的基因bexA。尽管最初的重组构建体包含其他开放阅读框，但仅bexA的存在就足以使大肠杆菌宿主对诺氟沙星、环丙沙星和溴化乙锭的耐药水平升高。嗜热栖热放线菌中bexA的破坏使该菌株对诺氟沙星、环丙沙星和溴化乙锭更敏感，表明该基因在这种生物体中表达并作为多药外排泵发挥作用。推导的BexA蛋白序列与多药外排转运蛋白副溶血性弧菌NorM的蛋白序列同源，因此，BexA属于多药和有毒化合物外排（MATE）家族。
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