大肠杆菌K-12中exuT基因转录方向的确定：exuT-uxaCA操纵子的双向转录
Determination of the transcription direction of the exuT gene in Escherichia coli K-12: divergent transcription of the exuT-uxaCA operons.
作者信息
Hugouvieux-Cotte-Pattat N, Robert-Baudouy J
出版信息
J Bacteriol. 1982 Jul;151(1):480-4. doi: 10.1128/jb.151.1.480-484.1982.
Abstract
The exuT gene of Escherichia coli, coding for the hexuronate transport system, was fused to lac genes by the use of Mu d(Apr lac) insertions (M. J. Casadaban and S. Cohen, Proc. Natl. Acad. Sci. U.S.A. 76:4530-4533, 1979). The method of chromosome mobilization with F' lac::Mu episomes (J. B. Zeldis, A. I. Bukhari, and D. Zipser, Virology 55:289-294, 1974) made it possible to determine the transcription direction of the exuT gene from the orientation of the Mu d(Apr lac) insertion in the fusion strains. Our results for a exuT-lac fusion strain suggest that the direction of transcription of other single gene operon exuT is clockwise on the standard E. coli map and confirm that the direction of transcription of uxaC is counterclockwise. The two close operons exuT and uxaCA are thus transcribed in opposite directions.
摘要
大肠杆菌中编码己糖醛酸转运系统的exuT基因，通过使用Mu d（Apr lac）插入片段与lac基因融合（M. J. 卡萨达班和S. 科恩，《美国国家科学院院刊》76:4530 - 4533, 1979）。利用F' lac::Mu附加体进行染色体迁移的方法（J. B. 泽尔迪斯、A. I. 布哈里和D. 齐普泽，《病毒学》55:289 - 294, 1974）使得从融合菌株中Mu d（Apr lac）插入片段的方向确定exuT基因的转录方向成为可能。我们对一个exuT - lac融合菌株的研究结果表明，在标准大肠杆菌图谱上，其他单基因操纵子exuT的转录方向是顺时针的，并证实uxaC的转录方向是逆时针的。因此，两个紧密相邻的操纵子exuT和uxaCA以相反的方向转录。
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本文引用的文献
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