大肠杆菌中新型基因座alx的碱性诱导
Alkaline induction of a novel gene locus, alx, in Escherichia coli.
作者信息
Bingham R J, Hall K S, Slonczewski J L
机构信息
Department of Biology, Kenyon College, Gambier, Ohio 43022.
出版信息
J Bacteriol. 1990 Apr;172(4):2184-6. doi: 10.1128/jb.172.4.2184-2186.1990.
Abstract
A novel pH-regulated locus inducible over 100-fold in alkaline media was identified in Escherichia coli through screening of 93,000 Mu dI1734 (lacZ Kmr) operon fusions at pH 6.5 and pH 8.5. Four lacZ fusions that showed expression only at the higher pH were mapped at 67.5 min by P1 transduction crosses. The locus was designated alx.
摘要
通过在pH 6.5和pH 8.5条件下筛选93,000个Mu dI1734（lacZ Kmr）操纵子融合体，在大肠杆菌中鉴定出一个在碱性培养基中诱导表达可超过100倍的新型pH调节位点。通过P1转导杂交将四个仅在较高pH下显示表达的lacZ融合体定位在67.5分钟处。该位点被命名为alx。
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