Physical mapping of mutations in the structural gene encoding for the Escherichia coli aldohexuronate transport system.

We used a convenient plasmid recombination procedure to map within the exuT gene, the structural gene for the aldohexuronate transport system, the regional locations of ten independent mutations which affect the transport activity. A set of six plasmids containing various portions of the exuT gene was constructed which allowed us to divide this gene into five parts.