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大肠杆菌醛糖己糖醛酸转运系统结构基因中突变的物理图谱分析。

Physical mapping of mutations in the structural gene encoding for the Escherichia coli aldohexuronate transport system.

作者信息

Mata-Gilsinger M, Ritzenthaler P

出版信息

Mol Gen Genet. 1983;189(2):355-7. doi: 10.1007/BF00337832.

DOI:10.1007/BF00337832
PMID:6343797
Abstract

We used a convenient plasmid recombination procedure to map within the exuT gene, the structural gene for the aldohexuronate transport system, the regional locations of ten independent mutations which affect the transport activity. A set of six plasmids containing various portions of the exuT gene was constructed which allowed us to divide this gene into five parts.

摘要

我们采用了一种简便的质粒重组程序,以定位醛糖醛酸转运系统的结构基因exuT基因内影响转运活性的10个独立突变的区域位置。构建了一组包含exuT基因不同部分的六个质粒,这使我们能够将该基因分为五个部分。

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1
Physical mapping of mutations in the structural gene encoding for the Escherichia coli aldohexuronate transport system.大肠杆菌醛糖己糖醛酸转运系统结构基因中突变的物理图谱分析。
Mol Gen Genet. 1983;189(2):355-7. doi: 10.1007/BF00337832.
2
Physiological and genetic regulation of the aldohexuronate transport system in Escherichia coli.大肠杆菌中醛己糖醛酸转运系统的生理和遗传调控
J Bacteriol. 1976 Aug;127(2):706-18. doi: 10.1128/jb.127.2.706-718.1976.
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Physical mapping of the exuT and uxaC operators by use of exu plasmids and generation of deletion mutants in vitro.利用exu质粒对exuT和uxaC操纵基因进行物理图谱分析并在体外构建缺失突变体。
J Bacteriol. 1983 Sep;155(3):973-82. doi: 10.1128/jb.155.3.973-982.1983.
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Isolation and characterization of Erwinia chrysanthemi mutants defective in degradation of hexuronates.菊欧文氏菌己糖醛酸降解缺陷型突变体的分离与鉴定
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Determination of the transcription direction of the exuT gene in Escherichia coli K-12: divergent transcription of the exuT-uxaCA operons.大肠杆菌K-12中exuT基因转录方向的确定:exuT-uxaCA操纵子的双向转录
J Bacteriol. 1982 Jul;151(1):480-4. doi: 10.1128/jb.151.1.480-484.1982.
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Isolation of specialized transducing bacteriophages carrying the structural genes of the hexuronate system in Escherichia coli K-12: exu region.在大肠杆菌K-12中携带己糖醛酸系统结构基因的特异性转导噬菌体的分离:exu区域
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Front Microbiol. 2020 Jan 23;11:27. doi: 10.3389/fmicb.2020.00027. eCollection 2020.

本文引用的文献

1
Molecular cloning of Escherichia coli K-12 hexuronate system genes: exu region.大肠杆菌K-12己糖醛酸系统基因的分子克隆:exu区域
J Bacteriol. 1981 Jan;145(1):181-90. doi: 10.1128/jb.145.1.181-190.1981.
2
Determination of the transcription direction of the exuT gene in Escherichia coli K-12: divergent transcription of the exuT-uxaCA operons.大肠杆菌K-12中exuT基因转录方向的确定:exuT-uxaCA操纵子的双向转录
J Bacteriol. 1982 Jul;151(1):480-4. doi: 10.1128/jb.151.1.480-484.1982.
3
A modified pBR322 vector with improved properties for the cloning, recovery, and sequencing of blunt-ended DNA fragments.
一种经过改良的pBR322载体,具有用于平端DNA片段克隆、回收和测序的改进特性。
Gene. 1982 Feb;17(2):189-96. doi: 10.1016/0378-1119(82)90072-5.
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Calcium-dependent bacteriophage DNA infection.钙依赖性噬菌体DNA感染。
J Mol Biol. 1970 Oct 14;53(1):159-62. doi: 10.1016/0022-2836(70)90051-3.
5
Construction of biologically functional bacterial plasmids in vitro.体外构建具有生物学功能的细菌质粒。
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4. doi: 10.1073/pnas.70.11.3240.
6
Sensitization of D-glucuronic acid transport system of E. coli to protein group reagents in presence of substrate or absence of energy source.在有底物存在或无能源的情况下,大肠杆菌D-葡萄糖醛酸转运系统对蛋白质类试剂的敏感性。
Biochem Biophys Res Commun. 1973 Oct 15;54(4):1342-6. doi: 10.1016/0006-291x(73)91134-0.
7
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.利用分析型琼脂糖-溴化乙锭电泳检测副流感嗜血杆菌中的两种限制性内切酶活性。
Biochemistry. 1973 Jul 31;12(16):3055-63. doi: 10.1021/bi00740a018.
8
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳测定小双链和单链DNA分子的链长
Biochemistry. 1975 Aug 26;14(17):3787-94. doi: 10.1021/bi00688a010.
9
Physiological and genetic regulation of the aldohexuronate transport system in Escherichia coli.大肠杆菌中醛己糖醛酸转运系统的生理和遗传调控
J Bacteriol. 1976 Aug;127(2):706-18. doi: 10.1128/jb.127.2.706-718.1976.
10
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.一种用于筛选重组质粒DNA的快速碱性提取方法。
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23. doi: 10.1093/nar/7.6.1513.