通过用含有少量蛋白质的聚丙烯酰胺凝胶切片直接免疫产生的特异性抗血清。

Specific antisera produced by direct immunization with slices of polyacrylamide gel containing small amounts of protein.

作者信息

Boulard C, Lecroisey A

出版信息

J Immunol Methods. 1982;50(2):221-6. doi: 10.1016/0022-1759(82)90228-9.

Abstract

Rabbits were injected with slices of polyacrylamide gels containing entrapped insect proteins after separation by electrophoresis. Specific antibodies were produced independently of the nature of the gel (with or without sodium dodecyl sulphate) and of the staining technique (amido black or Coomassie Blue). The procedure appears to be a rapid and simple method for production of antibodies specific to proteins separated in minute quantities from a complex mixture.

摘要

兔子在聚丙烯酰胺凝胶切片经电泳分离后被注射，这些凝胶切片中含有截留的昆虫蛋白。特异性抗体的产生与凝胶的性质（有无十二烷基硫酸钠）和染色技术（氨基黑或考马斯亮蓝）无关。该方法似乎是一种快速简便的方法，用于制备针对从复杂混合物中微量分离出的蛋白质的特异性抗体。