禽成髓细胞瘤病毒转化基因与至少由一个间隔序列分隔的独特鸡DNA区域相关。
Avian myeloblastosis virus transforming gene is related to unique chicken DNA regions separated by at least one intervening sequence.
作者信息
Perbal B, Baluda M A
出版信息
J Virol. 1982 Jan;41(1):250-7. doi: 10.1128/JVI.41.1.250-257.1982.
Abstract
Identification of several additional restriction endonuclease sites within the cellular substitution (amv) inserted into the avian myeloblastosis virus proviral genome has permitted us to isolate different regions of the amv sequence. These subsets of the avian myeloblastosis virus transforming gene have been cloned in the plasmid pBR322 and used as hybridization probes to investigate the topology of homologous (proto-amv) normal chicken DNA sequences. The results showed that the cellular proto-amv sequences in C/O chicken DNA are interrupted by at least one intervening sequence. A partial arrangement of the proto-amv sequences is presented.
摘要
对插入禽成髓细胞瘤病毒前病毒基因组中的细胞替代物（amv）内几个额外的限制性内切酶位点进行鉴定，使我们能够分离出amv序列的不同区域。禽成髓细胞瘤病毒转化基因的这些亚组已克隆到质粒pBR322中，并用作杂交探针来研究同源（原amv）正常鸡DNA序列的拓扑结构。结果表明，C/O鸡DNA中的细胞原amv序列被至少一个间隔序列中断。本文给出了原amv序列的部分排列。
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