Quaedvlieg N, Dockx J, Keultjes G, Kock P, Wilmering J, Weisbeek P, Smeekens S
Department of Molecular Cell Biology, University of Utrecht, Netherlands.
Plant Mol Biol. 1996 Dec;32(5):987-93. doi: 10.1007/BF00020495.
Seven different MYB-related genes have been isolated from a genomic Arabidopsis library with probes based on MYB DNA-binding motifs. The predicted amino acid sequence of these genes showed high similarity in the MYB domain but outside this region virtually no similarities were found. The set of MYB-related genes was used to identify differentially expressed genes following the transfer of etiolated seedlings to light. This differential screen resulted in the selection of the ATM4 gene which is induced by light within one hour of exposure of etiolated or dark-adapted seedlings.
利用基于MYB DNA结合基序的探针,从拟南芥基因组文库中分离出7个不同的MYB相关基因。这些基因的预测氨基酸序列在MYB结构域显示出高度相似性,但在该区域之外几乎没有发现相似性。利用这组MYB相关基因来鉴定黄化幼苗转移到光照下后差异表达的基因。这种差异筛选导致选择了ATM4基因,该基因在黄化或暗适应的幼苗暴露于光照后1小时内被光诱导。