用生物测定系统研究嗜异性小鼠白血病病毒中的Fv-1决定簇：以隐匿形式分离具有N-嗜性Fv-1活性的嗜异性病毒。
Fv-1 determinants in xenotropic murine leukemia viruses studied with biological assay systems: Isolation of xenotropic virus with N-tropic Fv-1 activity in the cryptic form.
作者信息
Sakai K, Narita H, Adachi A, Tsuruta S, Yorifuji T, Ishimoto A
出版信息
J Virol. 1982 Apr;42(1):331-6. doi: 10.1128/JVI.42.1.331-336.1982.
Abstract
By a biological assay system using phenotypically mixed ecotropic and xenotropic murine leukemia viruses, we investigated whether in the virions of a xenotropic virus there is N- or B-tropic Fv-1 determinant in active form. The existence of N-tropic Fv-1 determinant was demonstrated in SL-XT-1 xenotropic virus isolated from the spleen of a 3-month-old SL mouse, and the N-tropic Fv-1 tropism was confirmed by analysis of the phenotypically mixed viruses harvested from clonal SC-1 cells doubly infected with the SL-XT-1 and B-tropic ecotropic viruses. However, neither N- nor B-tropic Fv-1 determinant was demonstrated in any xenotropic viruses isolated from embryo cells of BALB/c, NZB, or DBA/2 mice, or Cas E #1-IU, and xenotropic-like virus isolated from a wild mouse.
摘要
通过使用表型混合的亲嗜性和异嗜性鼠白血病病毒的生物测定系统，我们研究了在异嗜性病毒的病毒粒子中是否存在活性形式的 N 型或 B 型亲嗜性 Fv-1 决定簇。在从 3 个月大的 SL 小鼠脾脏中分离出的 SL-XT-1 异嗜性病毒中证实了 N 型亲嗜性 Fv-1 决定簇的存在，并且通过分析从双重感染了 SL-XT-1 和 B 型亲嗜性亲嗜性病毒的克隆 SC-1 细胞中收获的表型混合病毒，证实了 N 型亲嗜性 Fv-1 嗜性。然而，在从 BALB/c、NZB 或 DBA/2 小鼠的胚胎细胞中分离出的任何异嗜性病毒、Cas E #1-IU 以及从野生小鼠中分离出的类异嗜性病毒中，均未证实存在 N 型或 B 型亲嗜性 Fv-1 决定簇。
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