Galiègue S, Bailleul B, Loucheux-Lefebvre M H, Laval J
Carcinogenesis. 1982;3(4):435-8. doi: 10.1093/carcin/3.4.435.
Purified DNA was modified in vitro by 3H-labelled O-acetyl or O,O'-diacetyl-4-hydroxyaminoquinoline-1-oxide (Ac4HAQO or di Ac-4HAQO). It was then subjected to the action of the single-stranded DNA specific nuclease S1 and the digested fractions were analysed. For both types of modified DNA, the release of non-modified nucleotides was faster than the release of modified nucleotides. This result is at variance with that obtained with acetoxy-acetylaminofluorene-modified DNA: in the latter case, the modified nucleotides were preferentially released. The results suggest that the S1 endonuclease can recognize different conformational changes in DNA, which depend on the carcinogen used. The enzymatic activity (or activities) present in Micrococcus luteus cell extracts released ethanol-soluble products from Ac-4HAQO modified DNA.
纯化的DNA在体外被3H标记的O - 乙酰基或O，O'-二乙酰基 - 4 - 羟基氨基喹啉 - 1 - 氧化物（Ac4HAQO或二乙酰基 - 4HAQO）修饰。然后使其经受单链DNA特异性核酸酶S1的作用，并对消化后的组分进行分析。对于两种类型的修饰DNA，未修饰核苷酸的释放比修饰核苷酸的释放更快。该结果与用乙酰氧基 - 乙酰氨基芴修饰的DNA所获得的结果不同：在后一种情况下，修饰的核苷酸优先释放。结果表明，S1核酸内切酶可以识别DNA中不同的构象变化，这取决于所使用的致癌物。藤黄微球菌细胞提取物中存在的一种或多种酶活性从Ac - 4HAQO修饰的DNA中释放出乙醇溶性产物。
