Galiègue S, Lecocq G, Loucheux-Lefebvre M H
Biochim Biophys Acta. 1980 Oct 17;609(3):383-91. doi: 10.1016/0005-2787(80)90112-4.
The diacetyl derivative of 4-hydroxyaminoquinoline 1-oxide, the proximate carcinogen of 4-nitroquinoline 1-oxide, was reacted in vitro with native and heat denatured chicken erythrocyte DNA under various conditions. The amount of fixed carcinogen was obtained by using the labeled diacetyl derivative and from this result the molar extinction coefficients of bound carcinogen were calculated in order to allow a direct spectrophotometric determination. A decrease in melting temperature of DNA samples modified by O,O'-diacetyl 4-hydroxyaminoquinoline 1-oxide (di Ac-4HAQO) was measured: the melting temperature depression value is equal to 1.4 degrees C per 1% of modified DNA bases. This result was compared with the values previously obtained by Fuchs and coworkers (Fuchs, R. and Daune, M. (1973) FEBS Lett, 34, 295-298 and Fuchs, R. Lefebvre, J.F., Pouyet, J. and Daune, M. (1976) Biochemistry 15, 3347-3351) for N-acetoxy-N-2-acetylaminofluorene-modified DNA and by Lang et al. [25] for the phenanthrylation of the DNA bases of N-acetoxy-N-2-acetylaminophenanthrene-modified DNA.
4-羟基氨基喹啉1-氧化物的二乙酰衍生物是4-硝基喹啉1-氧化物的直接致癌物，在各种条件下与天然和热变性的鸡红细胞DNA在体外发生反应。通过使用标记的二乙酰衍生物获得固定致癌物的量，并根据该结果计算结合致癌物的摩尔消光系数，以便进行直接分光光度测定。测定了经O,O'-二乙酰基4-羟基氨基喹啉1-氧化物（二乙酰基-4HAQO）修饰的DNA样品的解链温度降低：每1%修饰的DNA碱基，解链温度降低值等于1.4℃。将该结果与Fuchs及其同事（Fuchs, R.和Daune, M.（1973年）《欧洲生物化学学会联合会快报》，34，295 - 298以及Fuchs, R.、Lefebvre, J.F.、Pouyet, J.和Daune, M.（1976年）《生物化学》，15，3347 - 3351）先前获得的N-乙酰氧基-N-2-乙酰氨基芴修饰的DNA的值以及Lang等人[25]先前获得的N-乙酰氧基-N-2-乙酰氨基菲修饰的DNA的碱基菲啶化的值进行了比较。
