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T抗原和VP1基因转位的猿猴病毒40突变体。

Simian virus 40 mutant with transposed T-antigen and VP1 genes.

作者信息

Lazarowitz S G

出版信息

J Virol. 1982 Mar;41(3):1025-37. doi: 10.1128/JVI.41.3.1025-1037.1982.

DOI:10.1128/JVI.41.3.1025-1037.1982
PMID:6284959
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256840/
Abstract

A simian virus 40 mutant with the T-antigen gene transposed to the late region of the viral genome has been constructed. This transposed molecule directed the synthesis of a full-sized, 92,000-dalton large T antigen in both permissive and nonpermissive cells. This large T antigen functioned in the initiation of viral DNA replication and in the transformation of nonpermissive cells. T-antigen synthesis by this transposed genome had the characteristics of late transcription, thus indicating that functional large T antigen of simian virus 40 is not required for the initiation of late transcription.

摘要

已构建出一种猿猴病毒40（SV40）突变体，其T抗原基因转座至病毒基因组的晚期区域。这种转座分子在允许细胞和非允许细胞中均能指导合成全长的、92,000道尔顿的大T抗原。这种大T抗原在病毒DNA复制起始以及非允许细胞的转化过程中发挥作用。由这种转座基因组合成的T抗原具有晚期转录的特征，因此表明猿猴病毒40功能性大T抗原对于晚期转录的起始并非必需。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/83592ae355df/jvirol00162-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/502a66e65a40/jvirol00162-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/e4fc6b0b13c2/jvirol00162-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/a61840b23f69/jvirol00162-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/5b021cc4165d/jvirol00162-0298-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/fb5b84cb743a/jvirol00162-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/a5dfea93a438/jvirol00162-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/83592ae355df/jvirol00162-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/502a66e65a40/jvirol00162-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/e4fc6b0b13c2/jvirol00162-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/a61840b23f69/jvirol00162-0297-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/5b021cc4165d/jvirol00162-0298-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/fb5b84cb743a/jvirol00162-0299-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/a5dfea93a438/jvirol00162-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8243/256840/83592ae355df/jvirol00162-0301-a.jpg

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DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE.免疫荧光法检测SV40转化细胞中的特异性抗原
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