鉴定羟甲基三甲基补骨脂素与RNA共价光加成产生的修饰核苷酸。
Identification of the modified nucleotides produced by covalent photoaddition of hydroxymethyltrimethylpsoralen to RNA.
作者信息
Bachellerie J P, Thompson J F, Wegnez M R, Hearst J E
出版信息
Nucleic Acids Res. 1981 May 11;9(9):2207-22. doi: 10.1093/nar/9.9.2207.
Abstract
The reaction between RNA and 4'hydroxymethyl-4,5',8-trimethylpsoralen has been studied. Both natural RNA and synthetic RNAs were used. The base specificity of the reaction was found to be the same in natural RNA, homopolymers, and mononucleotides. Uridine was found to be the most reactive base in all cases. The kinetics of formation and reversal of monoadducts and crosslinks has been examined. Paper electrophoretic conditions are described which provide a separation of the monoaddition and crosslinked photoproducts. The relative and absolute amounts of monoadducts and crosslinks can be determined very accurately with this system. Paper electrophoresis provides good separations of the different photoproducts. The mobilities of the products are a simple function of their molecular weights and charges.
摘要
对RNA与4'-羟甲基-4,5',8-三甲基补骨脂素之间的反应进行了研究。使用了天然RNA和合成RNA。发现该反应在天然RNA、同聚物和单核苷酸中的碱基特异性相同。在所有情况下，尿苷都是反应活性最高的碱基。研究了单加合物和交联物形成与逆转的动力学。描述了纸电泳条件，该条件可实现单加成和交联光产物的分离。使用该系统可以非常准确地测定单加合物和交联物的相对和绝对量。纸电泳能很好地分离不同的光产物。产物的迁移率是其分子量和电荷的简单函数。
相似文献
1
Identification of the modified nucleotides produced by covalent photoaddition of hydroxymethyltrimethylpsoralen to RNA.鉴定羟甲基三甲基补骨脂素与RNA共价光加成产生的修饰核苷酸。
Nucleic Acids Res. 1981 May 11;9(9):2207-22. doi: 10.1093/nar/9.9.2207.
2
Identification of a site of psoralen crosslinking in E. coli 16S ribosomal RNA.大肠杆菌16S核糖体RNA中补骨脂素交联位点的鉴定。
Nucleic Acids Res. 1982 May 11;10(9):2839-49. doi: 10.1093/nar/10.9.2839.
3
Specificity of the photoreaction of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen with ribonucleic acid. Identification of reactive sites in Escherichia coli phenylalanine-accepting transfer ribonucleic acid.4'-（羟甲基）-4,5',8-三甲基补骨脂素与核糖核酸光反应的特异性。大肠杆菌苯丙氨酸接受性转移核糖核酸中反应位点的鉴定。
Biochemistry. 1982 Mar 16;21(6):1357-63. doi: 10.1021/bi00535a039.
4
Dependence of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen photoaddition on the conformation of ribonucleic acid.4'-(羟甲基)-4,5',8-三甲基补骨脂素光加成反应对核糖核酸构象的依赖性
Biochemistry. 1982 Mar 16;21(6):1363-8. doi: 10.1021/bi00535a040.
5
Characterization of the effects on ovalbumin mRNA of aminomethyl-trimethylpsoralen photoreaction with hen oviduct mRNA.氨甲基三甲基补骨脂素与鸡输卵管mRNA光反应对卵清蛋白mRNA影响的表征。
Nucleic Acids Res. 1987 Oct 26;15(20):8417-38. doi: 10.1093/nar/15.20.8417.
6
Properties of Escherichia coli 16S ribosomal ribonucleic acid treated with 4,5',8-trimethylpsoralen and light.经4,5',8-三甲基补骨脂素和光照处理的大肠杆菌16S核糖体核糖核酸的特性
Biochemistry. 1979 May 15;18(10):2012-9. doi: 10.1021/bi00577a027.
7
Structure of E. coli 16S RNA elucidated by psoralen crosslinking.通过补骨脂素交联阐明的大肠杆菌16S RNA结构。
Cell. 1983 Apr;32(4):1355-65. doi: 10.1016/0092-8674(83)90316-1.
8
Termination sites of the in vitro nick-translation reaction on DNA that had photoreacted with psoralen.与补骨脂素发生光反应的DNA上体外切口平移反应的终止位点。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5540-4. doi: 10.1073/pnas.80.18.5540.
9
Analysis of RNA secondary structure by photochemical reversal of psoralen crosslinks.通过补骨脂素交联的光化学逆转分析RNA二级结构。
Nucleic Acids Res. 1979 Oct 10;7(3):689-703. doi: 10.1093/nar/7.3.689.
10
Interstrand DNA crosslinking by 4,5'8-trimethylpsoralen plus monochromatic ultraviolet light. Studies by alkaline elution in mouse L1210 leukemia cells.4,5',8-三甲基补骨脂素加单色紫外线诱导的链间DNA交联。小鼠L1210白血病细胞碱性洗脱研究。
Biochim Biophys Acta. 1980 Nov 14;610(1):56-63. doi: 10.1016/0005-2787(80)90055-6.
引用本文的文献
1
Triplex formation by a psoralen-conjugated oligodeoxyribonucleotide containing the base analog 8-oxo-adenine.由含有碱基类似物8-氧代腺嘌呤的补骨脂素共轭寡脱氧核糖核苷酸形成三链体。
Nucleic Acids Res. 1996 Feb 15;24(4):730-6. doi: 10.1093/nar/24.4.730.
2
Secondary structure model of the Mason-Pfizer monkey virus 5' leader sequence: identification of a structural motif common to a variety of retroviruses.猴空泡病毒5'前导序列的二级结构模型：多种逆转录病毒共有的结构基序的鉴定
J Virol. 1995 Apr;69(4):2175-86. doi: 10.1128/JVI.69.4.2175-2186.1995.
3
Identification of a site of psoralen crosslinking in E. coli 16S ribosomal RNA.大肠杆菌16S核糖体RNA中补骨脂素交联位点的鉴定。
Nucleic Acids Res. 1982 May 11;10(9):2839-49. doi: 10.1093/nar/10.9.2839.
4
Structure and function of ribosomal RNA.核糖体RNA的结构与功能。
Biochem J. 1985 Jul 1;229(1):1-17. doi: 10.1042/bj2290001.
5
Characterization of the effects on ovalbumin mRNA of aminomethyl-trimethylpsoralen photoreaction with hen oviduct mRNA.氨甲基三甲基补骨脂素与鸡输卵管mRNA光反应对卵清蛋白mRNA影响的表征。
Nucleic Acids Res. 1987 Oct 26;15(20):8417-38. doi: 10.1093/nar/15.20.8417.
6
Interaction of psoralen-derivatized oligodeoxyribonucleoside methylphosphonates with synthetic DNA containing a promoter for T7 RNA polymerase.补骨脂素衍生的寡脱氧核糖核苷甲基膦酸酯与含有T7 RNA聚合酶启动子的合成DNA的相互作用。
Nucleic Acids Res. 1988 Nov 25;16(22):10681-97. doi: 10.1093/nar/16.22.10681.
7
Antibody-nucleic acid interactions. Antibodies to psoralen-modified RNA as probes of RNA structure.抗体 - 核酸相互作用。以补骨脂素修饰的RNA抗体作为RNA结构的探针。
Nucleic Acids Res. 1987 Nov 11;15(21):9057-73. doi: 10.1093/nar/15.21.9057.
8
4.5S RNA is encoded by hundreds of tandemly linked genes, has a short half-life, and is hydrogen bonded in vivo to poly(A)-terminated RNAs in the cytoplasm of cultured mouse cells.4.5S RNA由数百个串联连接的基因编码，半衰期短，且在体内与培养的小鼠细胞细胞质中聚腺苷酸化的RNA形成氢键。
Mol Cell Biol. 1986 May;6(5):1508-19. doi: 10.1128/mcb.6.5.1508-1519.1986.
9
The human immunodeficiency virus type 1 packaging signal and major splice donor region have a conserved stable secondary structure.1型人类免疫缺陷病毒包装信号和主要剪接供体区域具有保守的稳定二级结构。
J Virol. 1992 Jul;66(7):4144-53. doi: 10.1128/JVI.66.7.4144-4153.1992.
本文引用的文献
1
Photoreactions at 3655 Angstrom between pyrimidine bases and skin-photosensitizing furocoumarins.嘧啶碱基与皮肤光敏性呋喃香豆素在3655埃处的光反应。
Photochem Photobiol. 1967 Oct;6(10):711-9. doi: 10.1111/j.1751-1097.1967.tb08736.x.
2
[On the effective mechanism of the photodynamic furocumarine photoreaction of psoralen-(4-14C) with DNA, RNA, homopolynucleotides and nucleosides].[关于补骨脂素 -（4 - 14C）与DNA、RNA、同聚核苷酸和核苷的光动力呋喃香豆素光反应的作用机制]
Photochem Photobiol. 1967 May;6(5):341-54. doi: 10.1111/j.1751-1097.1967.tb08882.x.
3
Studies on the photo-C4-cyclo-addition reactions between skin-photosensitizing furocoumarins and nucleic acids.皮肤光敏性呋喃香豆素与核酸之间的光化学4-环加成反应研究。
Photochem Photobiol. 1970 Jan;11(1):27-35. doi: 10.1111/j.1751-1097.1970.tb05714.x.
4
Photosensitization of skin in vivo by furocoumarins (psoralens).补骨脂素（呋喃香豆素）对皮肤的体内光敏作用。
Biochim Biophys Acta. 1969 Nov 19;195(1):197-206. doi: 10.1016/0005-2787(69)90616-9.
5
A two-dimensional fractionation procedure for radioactive nucleotides.一种用于放射性核苷酸的二维分级分离程序。
J Mol Biol. 1965 Sep;13(2):373-98. doi: 10.1016/s0022-2836(65)80104-8.
6
Light-induced cross-linking of DNA in the presence of a furocoumarin (psoralen). Studies with phage lambda, Escherichia coli, and mouse leukemia cells.在呋喃香豆素（补骨脂素）存在的情况下，光诱导DNA交联。用λ噬菌体、大肠杆菌和小鼠白血病细胞进行的研究。
Biochim Biophys Acta. 1970 Sep 17;217(1):30-9. doi: 10.1016/0005-2787(70)90119-x.
7
Cross-linking of DNA in situ as a probe for chromatin structure.原位DNA交联作为染色质结构的一种探针
Science. 1976 Jul 2;193(4247):62-4. doi: 10.1126/science.935855.
8
Psoralen-DNA photoreaction: controlled production of mono- and diadducts with nanosecond ultraviolet laser pulses.补骨脂素 - DNA光反应：利用纳秒紫外激光脉冲控制单加合物和双加合物的产生。
Science. 1977 Aug 26;197(4306):906-8. doi: 10.1126/science.887929.
9
Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA.具有与DNA和RNA更高光反应活性的新型补骨脂素衍生物的合成与表征
Biochemistry. 1977 Mar 22;16(6):1058-64. doi: 10.1021/bi00625a005.
10
Photochemistry of cytosine derivatives. 1. Photochemistry of thymidylyl-(3' leads to 5')-deoxycytidine.胞嘧啶衍生物的光化学。1. 胸苷酰基-（3' 至 5'）-脱氧胞苷的光化学。
Biochemistry. 1978 Nov 14;17(23):4865-76. doi: 10.1021/bi00616a003.
Zotero 插件