Stiegler P, Carbon P, Zuker M, Ebel J P, Ehresmann C
Nucleic Acids Res. 1981 May 11;9(9):2153-72. doi: 10.1093/nar/9.9.2153.
Extensive studies in our laboratory using different ribonucleases resulted in valuable data on the topography of the E.coli 16S ribosomal RNA within the native 30S subunit, within partially unfolded 30S subunits, in the free state, and in association with individual ribosomal proteins. Such studies have precise details on the accessibility of certain residues and delineated highly accessible RNA regions. Furthermore, they provided evidence that the 16S rRNA is organized in its subunit into four distinct domains. A secondary structure model of the E.coli 16S rRNA has been derived from these topographical data. Additional information from comparative sequence analyses of the small ribosomal subunit RNAs from other species sequenced so far has been used.
我们实验室使用不同的核糖核酸酶进行了广泛研究,得到了关于大肠杆菌16S核糖体RNA在天然30S亚基内、部分展开的30S亚基内、游离状态下以及与单个核糖体蛋白结合时的拓扑结构的有价值数据。此类研究给出了某些残基可及性的精确细节,并描绘出了高度可及的RNA区域。此外,这些研究还证明16S rRNA在其亚基中被组织成四个不同的结构域。大肠杆菌16S rRNA的二级结构模型就是从这些拓扑数据推导出来的。我们还利用了迄今为止已测序的其他物种的小核糖体亚基RNA的比较序列分析的额外信息。