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鸡中δ-晶状体蛋白基因的组织方式。

Organization of delta-crystallin genes in the chicken.

作者信息

Yasuda K, Kondoh H, Okada T S, Nakajima N, Shimura Y

出版信息

Nucleic Acids Res. 1982 May 11;10(9):2879-91. doi: 10.1093/nar/10.9.2879.

DOI:10.1093/nar/10.9.2879
PMID:6285314
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320662/
Abstract

Double-stranded DNA was synthesized from delta-crystallin mRNA prepared from lens fibers of 15-day-old chick embryos and cloned at the Pst I site of the plasmid pBR322. Using the cloned cDNA and single-stranded cDNA as hybridization probes, a number of genomic DNA fragments containing delta-crystallin gene sequences have been cloned from the partial and complete EcoRI digests of chick brain DNA. One of the clones from the partial digests contains a DNA fragment that consists of four EcoRI fragments of 7.6 kb, 4.0 kb, 2.6 kb, and 0.8 kb. The gene sequences reside in the (5')7.6 kb - 0.8 kb - 4.0 kb (3') fragments. Electron microscopy has provided evidence that the cloned DNA fragment includes the entire gene sequences complementary to delta-crystallin mRNA except for the 3' terminal poly(A) tail, and that the delta-crystallin gene is interrupted by at least 13 intervening sequences. Another clone contains a genomic fragment that consists of two EcoRI fragments of 3.0 kb and 11 kb. The DNA fragment in the latter clone represents a different delta-crystallin gene, as judged by restriction endonuclease mapping and by electron microscopy.

摘要

双链DNA是由从15日龄鸡胚晶状体纤维制备的δ-晶体蛋白mRNA合成的，并克隆到质粒pBR322的Pst I位点。使用克隆的cDNA和单链cDNA作为杂交探针，从鸡脑DNA的部分和完全EcoRI消化物中克隆了许多含有δ-晶体蛋白基因序列的基因组DNA片段。部分消化物中的一个克隆包含一个DNA片段，该片段由7.6 kb、4.0 kb、2.6 kb和0.8 kb的四个EcoRI片段组成。基因序列位于(5')7.6 kb - 0.8 kb - 4.0 kb (3')片段中。电子显微镜提供了证据，表明克隆的DNA片段包含除3'末端聚(A)尾外与δ-晶体蛋白mRNA互补的整个基因序列，并且δ-晶体蛋白基因被至少13个间隔序列中断。另一个克隆包含一个基因组片段，该片段由3.0 kb和11 kb的两个EcoRI片段组成。通过限制性内切酶图谱分析和电子显微镜判断，后一个克隆中的DNA片段代表一个不同的δ-晶体蛋白基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/6cd0cb4b76d1/nar00378-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/65e631678be2/nar00378-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/e087a3006fa4/nar00378-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/255ed79cd303/nar00378-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/6cd0cb4b76d1/nar00378-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/65e631678be2/nar00378-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/e087a3006fa4/nar00378-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/255ed79cd303/nar00378-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e9d/320662/6cd0cb4b76d1/nar00378-0127-a.jpg

相似文献

1
Organization of delta-crystallin genes in the chicken.鸡中δ-晶状体蛋白基因的组织方式。
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Molecular cloning and partial characterization of delta-crystallin cDNA sequences in a bacterial plasmid.
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引用本文的文献

1
Stimulation of crystallin synthesis in embryonic brain cells in culture.培养的胚胎脑细胞中晶状体蛋白合成的刺激。
Rouxs Arch Dev Biol. 1986 Jan;195(1):15-21. doi: 10.1007/BF00444037.
2
Sequence of a complete chicken delta-crystallin cDNA.完整鸡δ-晶体蛋白cDNA的序列
Proc Natl Acad Sci U S A. 1984 May;81(9):2611-5. doi: 10.1073/pnas.81.9.2611.
3
The nucleotide sequence of a complete chicken delta-crystallin cDNA.完整鸡δ-晶状体蛋白cDNA的核苷酸序列。

本文引用的文献

1
Structural organization of a 17 KB segment of the alpha 2 collagen gene: evaluation by R loop mapping.α2胶原蛋白基因17千碱基片段的结构组织：通过R环作图进行评估。
Nucleic Acids Res. 1980 May 24;8(10):2241-53. doi: 10.1093/nar/8.10.2241.
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Chicken lens crystallin DNA sequences show at least two delta-crystallin genes.鸡晶状体晶状体蛋白DNA序列显示至少有两个δ-晶状体蛋白基因。
Nature. 1980 Mar 20;284(5753):234-8. doi: 10.1038/284234a0.
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Transcription and site-specific hypomethylation of the delta-crystallin genes in the embryonic chicken lens.
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Delta crystallins and their nucleic acids.δ-晶体蛋白及其核酸。
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Structural and functional evidence for differential promoter activity of the two linked delta-crystallin genes in the chicken.鸡体内两个相连的δ-晶状体蛋白基因启动子活性差异的结构和功能证据。
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8
Tissue-specific regulation of a chicken delta-crystallin gene in mouse cells: involvement of the 5' end region.鸡δ-晶体蛋白基因在小鼠细胞中的组织特异性调控：5'端区域的作用
EMBO J. 1985 Sep;4(9):2201-7. doi: 10.1002/j.1460-2075.1985.tb03915.x.
9
Nucleotide sequence of a chicken delta-crystallin gene.鸡δ-晶体蛋白基因的核苷酸序列。
Nucleic Acids Res. 1985 Mar 11;13(5):1593-606. doi: 10.1093/nar/13.5.1593.
10
Conservation of delta-crystallin gene structure between ducks and chickens.鸭和鸡之间δ-晶体蛋白基因结构的保守性。
J Mol Evol. 1987;25(4):308-17. doi: 10.1007/BF02603115.
胚胎期鸡晶状体中δ-晶体蛋白基因的转录及位点特异性低甲基化
J Biol Chem. 1981 Aug 10;256(15):8172-6.
4
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5
Organization and structure of an E. coli tRNA operon containing seven tRNA genes.包含七个tRNA基因的大肠杆菌tRNA操纵子的组织与结构。
Cell. 1981 Jan;23(1):239-49. doi: 10.1016/0092-8674(81)90288-9.
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Changes in chick lens proteins with aging.雏鸡晶状体蛋白质随衰老的变化。
Exp Eye Res. 1968 Oct;7(4):593-602. doi: 10.1016/s0014-4835(68)80014-4.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
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8
Macromolecular events during differentiation of the chicken lens.鸡晶状体分化过程中的大分子事件。
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