猿猴肉瘤病毒转化基因的核苷酸序列。
Nucleotide sequence of the transforming gene of simian sarcoma virus.
作者信息
Devare S G, Reddy E P, Robbins K C, Andersen P R, Tronick S R, Aaronson S A
出版信息
Proc Natl Acad Sci U S A. 1982 May;79(10):3179-82. doi: 10.1073/pnas.79.10.3179.
The sequence of the transforming region of simian sarcoma virus (SSV) has been determined by using molecularly cloned viral DNA. This region encompassed the 1.0-kilobase pair woolly monkey cell-derived insertion sequence, v-sis, and flanking simian sarcoma-associated viral (SSAV) sequences. A 675-nucleotide-long open reading frame commenced 19 nucleotides within the SSAV sequences to the left of the v-sis helper viral junction and terminated within v-sis itself. Possible promoter and acceptor splice signals were detected in helper viral sequences upstream from this open reading frame, and potential polyadenylylation sites were identified downstream both within v-sis and in helper viral sequences beyond v-sis. The recombinational event that led to the generation of SSV occurred in the middle of two functional codons, indicating that SSAV provided the regulatory elements for transcription as well as the initiation codon for translation of SSV cell-derived transforming sequences.
通过使用分子克隆的病毒DNA，已确定了猿猴肉瘤病毒（SSV）转化区的序列。该区域包括1.0千碱基对的绒毛猴细胞衍生插入序列v-sis，以及侧翼的猿猴肉瘤相关病毒（SSAV）序列。一个675个核苷酸长的开放阅读框在v-sis辅助病毒连接处左侧的SSAV序列内19个核苷酸处开始，并在v-sis本身内终止。在该开放阅读框上游的辅助病毒序列中检测到可能的启动子和受体剪接信号，并且在v-sis内以及v-sis之外的辅助病毒序列下游都鉴定出了潜在的聚腺苷酸化位点。导致SSV产生的重组事件发生在两个功能密码子的中间，这表明SSAV为转录提供了调控元件以及SSV细胞衍生转化序列翻译的起始密码子。
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