v-sis转化基因的体外诱变确定了其生长因子相关产物的功能结构域。
In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-related product.
作者信息
King C R, Giese N A, Robbins K C, Aaronson S A
出版信息
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5295-9. doi: 10.1073/pnas.82.16.5295.
The polypeptide sequence of the v-sis transforming gene product of simian sarcoma virus (SSV) can be divided into four regions that are likely to represent structural domains of the protein. Mutations were generated in the SSV nucleotide sequence to assay the extent or function of each of these regions. The results indicate that the helper virus-derived amino-terminal sequence as well as a core region homologous to polypeptide chain 2 of platelet-derived growth factor (PDGF) are required for the transforming function of the protein. Products of transforming but not nontransforming mutants formed dimer structures conformationally analogous to biologically active PDGF.
猿猴肉瘤病毒（SSV）的v-sis转化基因产物的多肽序列可分为四个区域，这些区域可能代表该蛋白质的结构域。在SSV核苷酸序列中产生突变，以分析这些区域中每个区域的功能范围。结果表明，该蛋白质的转化功能需要辅助病毒衍生的氨基末端序列以及与血小板衍生生长因子（PDGF）的多肽链2同源的核心区域。转化型而非非转化型突变体的产物形成了与生物活性PDGF构象类似的二聚体结构。
Zotero 插件