LaPorta R F, Taichman L B
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3393-7. doi: 10.1073/pnas.79.11.3393.
Human papilloma virus (HPV) is poorly understood because systems for its growth in tissue culture have not been developed. We report here that cultured human epidermal keratinocytes could be infected with HPV from plantar warts and that the viral DNA persisted and replicated as a stable episome. There were 50-200 copies of viral DNA per cell and there was no evidence to indicate integration of viral DNA into the cellular genome. There was also no evidence to suggest that viral DNA underwent productive replication. We conclude that cultured human epidermal keratinocytes may be a model for the study of certain aspects of HPV biology.
人乳头瘤病毒（HPV）尚未被充分了解，因为其在组织培养中的生长系统尚未开发出来。我们在此报告，培养的人表皮角质形成细胞可被来自跖疣的HPV感染，并且病毒DNA作为稳定的附加体持续存在并复制。每个细胞有50 - 200个病毒DNA拷贝，没有证据表明病毒DNA整合到细胞基因组中。也没有证据表明病毒DNA进行了有效复制。我们得出结论，培养的人表皮角质形成细胞可能是研究HPV生物学某些方面的一个模型。
