Adams J, Rothman E D
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3560-4. doi: 10.1073/pnas.79.11.3560.
The distribution of cleavage sites and their related sequences have been analyzed for 54 restriction endonucleases in the genome of human mitochondrial DNA; in three papova viruses, BK, simian virus 40, and polyoma; and in three bacteriophages, phi X174, fd, and G4. The results show that the cleavage sites and related sequences for most of the restriction enzymes tested are distributed nonrandomly. These results (i) constitute prima facie evidence for the action of natural selection, either direct or indirect on the restriction sites, and (ii) suggest that estimates of phylogenetic relationship, based on a phenetic approach using restriction enzyme data, will be biased.
已对人类线粒体DNA基因组中的54种限制性内切酶、三种乳头瘤病毒(BK病毒、猴病毒40和多瘤病毒)以及三种噬菌体(φX174、fd和G4)的切割位点及其相关序列进行了分析。结果表明,所测试的大多数限制性酶的切割位点和相关序列呈非随机分布。这些结果(i)构成了自然选择对限制位点直接或间接作用的初步证据,(ii)表明基于使用限制酶数据的表型方法对系统发育关系的估计将存在偏差。