Russnak R H, Jones D, Candido E P
Nucleic Acids Res. 1983 May 25;11(10):3187-205. doi: 10.1093/nar/11.10.3187.
The nucleotide sequences of two different cDNAs, CEHS48 and CEHS41, coding for the 16,000 dalton heat shock proteins (hsps) of Caenorhabditis elegans have been determined. CEHS48 codes for a polypeptide of 135 amino acids, approximately 15 fewer than the complete protein while CEHS41 is missing approximately 46 amino acids. From nucleotide 113 to the TAA termination signal the extent of homology between the sequences is 91%. Toward the 5' ends, the homology drops to 20% and results in completely divergent amino acid sequences. The 3' noncoding regions are only 30% homologous. Only CEHS48 contains a poly(A) signal and a poly(A) tail, suggesting that CEHS41 has an incomplete 3' end. The region from amino acid 43 to amino acid 115 shows extensive homology with corresponding regions in the four small hsps of Drosophila melanogaster and in mammalian alpha-crystallin. Two-dimensional gel analysis of in vitro synthesized hsp16 reveals the existence of five distinct components of identical molecular weights, but with different isoelectric points.
已测定了编码秀丽隐杆线虫16000道尔顿热休克蛋白（hsps）的两种不同cDNA（CEHS48和CEHS41）的核苷酸序列。CEHS48编码一个135个氨基酸的多肽，比完整蛋白质少约15个氨基酸，而CEHS41缺失约46个氨基酸。从核苷酸113到TAA终止信号，序列之间的同源性程度为91%。在5'端方向，同源性降至20%，并导致氨基酸序列完全不同。3'非编码区的同源性仅为30%。只有CEHS48含有一个聚腺苷酸化信号和一个聚腺苷酸尾，这表明CEHS41的3'端不完整。从氨基酸43到氨基酸115的区域与黑腹果蝇的四种小热休克蛋白以及哺乳动物α-晶体蛋白中的相应区域显示出广泛的同源性。对体外合成的hsp16进行二维凝胶分析，揭示了存在五个分子量相同但等电点不同的不同组分。
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