McCoy B J, Cohen I K
Connect Tissue Res. 1982;9(3):181-5. doi: 10.3109/03008208209160259.
Trypsin-activated collagenase activity was analyzed in media from keloid biopsies as well as keloid fibroblasts using radiolabeled guinea pig skin collagen as a substrate for collagenase digestion. Collagenase activity per tissue dry weight and DNA was similar in keloid and normal dermal biopsies; however, it was significantly elevated in keloid compared to normal dermal fibroblast media. In two paired cell lines where keloid and normal dermal fibroblasts were isolated from the same patient, relative collagen synthesis and trypsin-activated collagenase were increased to a similar extent in the keloid cell lines compared to normals. The biopsy data are in agreement with other findings, and further confirm the hypothesis that increased collagen deposition in keloids does not result from decreased collagen degradation. In vitro, however, the data presented here indicate that in some cell lines increased collagen synthesis may be associated with increased trypsin-activated collagenase.
以放射性标记的豚鼠皮肤胶原蛋白为胶原酶消化的底物，分析瘢痕疙瘩活检组织以及瘢痕疙瘩成纤维细胞培养基中的胰蛋白酶激活的胶原酶活性。瘢痕疙瘩和正常皮肤活检组织中每单位组织干重和DNA的胶原酶活性相似；然而，与正常皮肤成纤维细胞培养基相比，瘢痕疙瘩中的胶原酶活性显著升高。在从同一患者分离出瘢痕疙瘩和正常皮肤成纤维细胞的两个配对细胞系中，与正常细胞系相比，瘢痕疙瘩细胞系中的相对胶原合成和胰蛋白酶激活的胶原酶增加到相似程度。活检数据与其他研究结果一致，进一步证实了瘢痕疙瘩中胶原沉积增加并非由胶原降解减少所致的假说。然而，体外实验数据表明，在一些细胞系中，胶原合成增加可能与胰蛋白酶激活的胶原酶增加有关。
