Clore J N, Cohen I K, Diegelmann R F
Biochim Biophys Acta. 1979 Aug 22;586(2):384-90. doi: 10.1016/0304-4165(79)90107-7.
Molecular sieve column chromatography was used to determine the amount of type I and III collagen synthesized by normal dermis and keloid biopsies and fibroblasts derived from these tissues. After incubation with radioactive proline, the collagen was extracted and separated into types I and III and then quantitated. There was no significant difference in the percent type III collagen synthesized by fresh keloid biopsies compared to normal dermis. Likewise, there was no significant difference in the percent type III collagen synthesized by keloid fibroblasts compared to normal dermal fibroblasts. However, fibroblasts from both keloid and normal dermis synthesized a lower percentage of type III collagen in cell culture compared to the original biopsies. These findings demonstrate that keloid collagen has the same type distribution as normal dermis and suggest that increased collagen synthesis in these lesions is not related to altered collagen types.
采用分子筛柱色谱法测定正常真皮和瘢痕疙瘩活检组织以及源自这些组织的成纤维细胞合成的Ⅰ型和Ⅲ型胶原蛋白的量。在用放射性脯氨酸孵育后，提取胶原蛋白并分离为Ⅰ型和Ⅲ型，然后进行定量分析。与正常真皮相比，新鲜瘢痕疙瘩活检组织合成的Ⅲ型胶原蛋白百分比无显著差异。同样，与正常真皮成纤维细胞相比，瘢痕疙瘩成纤维细胞合成的Ⅲ型胶原蛋白百分比也无显著差异。然而，与原始活检组织相比，瘢痕疙瘩和成纤维细胞在细胞培养中合成的Ⅲ型胶原蛋白百分比均较低。这些发现表明，瘢痕疙瘩胶原蛋白的类型分布与正常真皮相同，提示这些病变中胶原蛋白合成增加与胶原蛋白类型改变无关。
