Goldblum S E, Reed W P
Infect Immun. 1982 Jul;37(1):336-43. doi: 10.1128/iai.37.1.336-343.1982.
Pneumococci have been shown to induce granulocytopenia and pulmonary leukostasis which might contribute to morbidity and mortality in pneumococcal sepsis. We studied whether other gram-positive species (groups A and B streptococci, Staphylococcus aureus, Bacillus cereus, and Clostridium perfringens) could also induce these phenomena. Rabbits were challenged with nonviable preparations of each species, and all five species induced profound granulocytopenia (mean decrease of 90%) and pulmonary leukostasis. In vitro studies of serum incubated with these species of bacteria showed a mean consumption of total hemolytic complement of 90%, a mean increase of chemotactic activity for granulocytes of 204%, and a mean augmentation of granulocyte adherence of 45% (compared with 18% for the control). Infusion of sonicate-exposed sera induced granulocytopenia in recipient rabbits. Thus, several nonviable gram-positive species can interact with serum to activate the complement system, generate C5a bioactivity, augment granulocyte adherence, and generate a neutropenia-inducing factor. These alterations may contribute to granulocytopenia or pulmonary leukostasis, which may play a role in the morbidity and mortality associated with gram-positive bacterial infections.
肺炎球菌已被证明可导致粒细胞减少和肺白细胞淤滞，这可能是肺炎球菌败血症发病和死亡的原因。我们研究了其他革兰氏阳性菌（A组和B组链球菌、金黄色葡萄球菌、蜡样芽孢杆菌和产气荚膜梭菌）是否也能引发这些现象。用每种细菌的非活性制剂对兔子进行攻击，所有这五种细菌都导致了严重的粒细胞减少（平均下降90%）和肺白细胞淤滞。对与这些细菌种类一起孵育的血清进行的体外研究显示，总溶血补体平均消耗90%，粒细胞趋化活性平均增加204%，粒细胞黏附平均增加45%（对照组为18%）。输注经超声处理的血清会使受体兔子出现粒细胞减少。因此，几种非活性革兰氏阳性菌可与血清相互作用，激活补体系统，产生C5a生物活性，增强粒细胞黏附，并产生一种诱导中性粒细胞减少的因子。这些改变可能导致粒细胞减少或肺白细胞淤滞，这可能在革兰氏阳性菌感染相关的发病和死亡中起作用。
