Jutila C K, Jutila M A, Crowell R E, Chick T W, Van Epps D E, Reed W P
Research Service, Veterans Administration Medical Center, Albuquerque, New Mexico.
Inflammation. 1992 Apr;16(2):135-46. doi: 10.1007/BF00918953.
Leukopenia and pulmonary leukostasis are prominent features in patients succumbing to pneumococcal (PNC) infections. We examined mechanisms involved in recruitment of polymorphonuclear neutrophils (PMNs) into pulmonary capillaries and alveolae after PNC sonicate injection. We showed that by 15 min postinjection, PMN chemotactic activity was found in bronchoalveolar lavage (BAL) fluids and increased with time until the end point of the study at 90 min. Accompanying the increased chemotactic activity in BAL fluids was a decrease in circulating PMNs more pronounced in the femoral artery (FA) than the pulmonary artery (PA). Superoxide anion (O2-) production by peripheral PMNs was depressed following PNC sonicate injection, and comparison of FA and PA showed that FA PMNs produced less O2- than PA PMNs. PA PMNs also showed enhanced random migration when compared to the depressed random migration of FA PMNs. This study demonstrated that an intravascular challenge of PNC sonicate was associated with increased chemotactic activity for PMNs in BAL fluid. Fewer PMNs and altered PMN function resulted from passage through the pulmonary microvasculature after PNC sonicate injection.
白细胞减少和肺白细胞淤滞是死于肺炎球菌（PNC）感染患者的突出特征。我们研究了注射PNC超声裂解物后多形核中性粒细胞（PMN）募集到肺毛细血管和肺泡中的相关机制。我们发现，注射后15分钟，支气管肺泡灌洗（BAL）液中出现PMN趋化活性，并随时间增加，直至研究终点90分钟。随着BAL液中趋化活性增加，循环中的PMN减少，股动脉（FA）中的减少比肺动脉（PA）中更明显。注射PNC超声裂解物后，外周PMN的超氧阴离子（O2-）产生受到抑制，FA和PA的比较显示，FA中的PMN产生的O2-比PA中的少。与FA中PMN随机迁移受抑制相比，PA中的PMN随机迁移也增强。本研究表明，PNC超声裂解物的血管内刺激与BAL液中PMN趋化活性增加有关。PNC超声裂解物注射后，通过肺微血管导致PMN数量减少和功能改变。
