水痘-带状疱疹病毒基因组的内部结构。

The internal organization of the varicella-zoster virus genome.

作者信息

Gilden D H, Shtram Y, Friedmann A, Wellish M, Devlin M, Fraser N, Becker Y

出版信息

J Gen Virol. 1982 Jun;60(Pt 2):371-4. doi: 10.1099/0022-1317-60-2-371.

Abstract

DNA was extracted from varicella-zoster (VZ) virions prepared in sucrose gradients. Thirty-eight molecules examined by electron microscopy were found to have a mean length of 46.7 micrometers. Examination of self-annealed VZV DNA molecules revealed that the virus genome was composed of a unique linear large sequence with a mol. wt. of 74.4 X 10(6) to 78.4 X 10(6), and a unique short sequence of mol. wt. approx. 9.8 X 10(6) flanked by inverted repeat sequences of 4.7 X 10(6) mol. wt.

摘要

从蔗糖梯度中制备的水痘带状疱疹（VZ）病毒粒子中提取DNA。通过电子显微镜检查的38个分子的平均长度为46.7微米。对自退火的VZV DNA分子的检查表明，病毒基因组由一个独特的线性大序列组成，其分子量为74.4×10⁶至78.4×10⁶，以及一个独特的短序列，分子量约为9.8×10⁶，两侧是分子量为4.7×10⁶的反向重复序列。