用11种限制性内切酶构建的水痘-带状疱疹病毒DNA物理图谱。
Physical maps of varicella-zoster virus DNA derived with 11 restriction enzymes.
作者信息
Mishra L, Dohner D E, Wellinghoff W J, Gelb L D
出版信息
J Virol. 1984 May;50(2):615-8. doi: 10.1128/JVI.50.2.615-618.1984.
Abstract
Varicella-zoster virus DNA was digested with 11 restriction endonucleases, and the resulting fragments were separated on agarose gels. Terminal fragments were identified by lambda exonuclease digestion. Physical maps were then constructed using a combination of double restriction enzyme digestion and hybridization to cloned BamHI fragments to place the remaining fragments in order.
摘要
水痘带状疱疹病毒DNA用11种限制性内切酶进行消化，所得片段在琼脂糖凝胶上进行分离。通过λ外切核酸酶消化鉴定末端片段。然后结合使用双酶切和与克隆的BamHI片段杂交的方法构建物理图谱，以便对其余片段进行排序。
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