在实验动物中制备水痘带状疱疹病毒抗血清。简要报告。
The production of varicella Zoster virus antiserum in laboratory animals. Brief report.
作者信息
Wroblewska Z, Devlin M, Reilly K, van Trieste H, Wellish M, Gilden D H
出版信息
Arch Virol. 1982;74(2-3):233-8. doi: 10.1007/BF01314717.
Hyperimmune anti-varicella zoster virus (VZV) antisera were prepared in BALB/c mice, Lewis rats and New Zealand rabbits by subcutaneous inoculations of purified VZ virions suspended in Freund's adjuvant. VZV antibodies were demonstrated both by an enzyme-linked immunosorbent assay (ELISA) and by indirect immunofluorescence (IF). VZV could not be detected in organs of any inoculated animals by IF or by cocultivation of tissue fragments from infected animals with human diploid fibroblasts.
通过皮下接种悬浮于弗氏佐剂中的纯化水痘带状疱疹病毒（VZV）病毒粒子，在BALB/c小鼠、Lewis大鼠和新西兰兔中制备了超免疫抗VZV抗血清。通过酶联免疫吸附测定（ELISA）和间接免疫荧光法（IF）检测到了VZV抗体。通过IF或通过将感染动物的组织碎片与人二倍体成纤维细胞共培养，在任何接种动物的器官中均未检测到VZV。
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