Kost T A, Theodorakis N, Hughes S H
Nucleic Acids Res. 1983 Dec 10;11(23):8287-301. doi: 10.1093/nar/11.23.8287.
The nucleotide sequence of the chick beta-actin gene was determined. The gene contains 5 introns; 4 interrupt the translated region at codons 41/42, 120/122, 267, 327/328 and a large intron occurs in the 5' untranslated region. The gene has a 97 nucleotide 5'-untranslated region and a 594 nucleotide 3'-untranslated region. A slight heterogeneity in the position of the poly A addition site exists; polyadenylation can occur at either of two positions two nucleotides apart. The gene codes for an mRNA of 1814 or 1816 nucleotides, excluding the poly(A) tail. In contrast to the chick skeletal muscle actin gene the beta-actin gene lacks the Cys codon between the initiator ATG and the codon for the N-terminal amino acid of the mature protein. In the 5' flanking DNA, 15 nucleotides downstream from the CCAAT sequence, is a tract of 25 nucleotides that is highly homologous to the sequence found in the same region of the rat beta-actin gene.
测定了鸡β-肌动蛋白基因的核苷酸序列。该基因含有5个内含子；4个在内含子在密码子41/42、120/122、267、327/328处打断编码区，一个大的内含子出现在5'非翻译区。该基因有一个97个核苷酸的5'非翻译区和一个594个核苷酸的3'非翻译区。聚腺苷酸加尾位点的位置存在轻微异质性；聚腺苷酸化可在相隔两个核苷酸的两个位置中的任何一个发生。该基因编码一个1814或1816个核苷酸的mRNA，不包括聚（A）尾。与鸡骨骼肌肌动蛋白基因不同，β-肌动蛋白基因在起始ATG和成熟蛋白N端氨基酸密码子之间缺少半胱氨酸密码子。在5'侧翼DNA中，在CCAAT序列下游15个核苷酸处，有一段25个核苷酸的序列，与大鼠β-肌动蛋白基因同一区域的序列高度同源。
