聚（dA）的两种类似物的合成及物理化学性质：聚（2-氨基嘌呤-9-β-D-脱氧核糖核苷酸）和聚2-氨基脱氧腺苷酸。
Synthesis and physicochemical properties of two analogs of poly(dA): poly(2-aminopurine-9-beta-D-deoxyribonucleotide) and poly 2-amino-deoxyadenylic acid.
作者信息
Scheit K H, Rackwitz H R
出版信息
Nucleic Acids Res. 1982 Jul 10;10(13):4059-69. doi: 10.1093/nar/10.13.4059.
Abstract
Polymerization of chemically synthesized dn2h6ATP and dn2ATP by deoxynucleotidyl transferase from calf thymus furnished poly(dn2h6A) and poly(dn2A) respectively. The synthetic polynucleotides were characterized by spectroscopic, ultracentrifugation and enzymatic methods. In polynucleotide-polynucleotide interaction, poly(dn2h6A) and poly(dn2A) behaved like analogs of poly(dnA).
摘要
小牛胸腺脱氧核苷酸转移酶催化化学合成的二氢六聚脱氧腺苷三磷酸（dn2h6ATP）和二聚脱氧腺苷三磷酸（dn2ATP）分别聚合，得到聚（二氢六聚脱氧腺苷）（poly(dn2h6A)）和聚（二聚脱氧腺苷）（poly(dn2A)）。通过光谱、超速离心和酶促方法对合成的多核苷酸进行了表征。在多核苷酸-多核苷酸相互作用中，聚（二氢六聚脱氧腺苷）和聚（二聚脱氧腺苷）的行为类似于聚（脱氧腺苷）（poly(dnA)）的类似物。
相似文献
1
Synthesis and physicochemical properties of two analogs of poly(dA): poly(2-aminopurine-9-beta-D-deoxyribonucleotide) and poly 2-amino-deoxyadenylic acid.聚（dA）的两种类似物的合成及物理化学性质：聚（2-氨基嘌呤-9-β-D-脱氧核糖核苷酸）和聚2-氨基脱氧腺苷酸。
Nucleic Acids Res. 1982 Jul 10;10(13):4059-69. doi: 10.1093/nar/10.13.4059.
2
Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid.多核苷酸。XL。聚2'-叠氮基-2'-脱氧腺苷酸的合成及性质
Nucleic Acids Res. 1976 Aug;3(8):2089-99. doi: 10.1093/nar/3.8.2089.
3
Polynucleotides. XLVI. 1 Synthesis and properties of poly (2'-amino-2'-deoxyadenylic acid).多核苷酸。XLVI。1. 聚（2'-氨基-2'-脱氧腺苷酸）的合成与性质
Nucleic Acids Res. 1977 Apr;4(4):989-1000. doi: 10.1093/nar/4.4.989.
4
Polynucleotides. LII. Synthesis and properties of poly(2'-deoxy-2'-fluoroadenylic acid).多核苷酸。LII。聚（2'-脱氧-2'-氟腺苷酸）的合成与性质
Nucleic Acids Res. 1978 Jun;5(6):1877-87. doi: 10.1093/nar/5.6.1877.
5
Enzymatic synthesis of polymers containing nicotinamide mononucleotide.含烟酰胺单核苷酸的聚合物的酶促合成。
Nucleic Acids Res. 1995 Sep 25;23(18):3742-9. doi: 10.1093/nar/23.18.3742.
6
Physical studies of the interaction of a calf thymus helix-destablizing protein with nucleic acids.小牛胸腺解旋蛋白与核酸相互作用的物理学研究。
Biochemistry. 1980 Sep 30;19(20):4674-82. doi: 10.1021/bi00561a021.
7
8
Polynucleotides. L. Synthesis and properties of poly (2'-chloro-2'-deoxyadenylic acid) and poly (2'-bromo-2'-deoxyadenylic acid).多核苷酸。L.聚（2'-氯-2'-脱氧腺苷酸）和聚（2'-溴-2'-脱氧腺苷酸）的合成及性质
Nucleic Acids Res. 1977 Dec;4(12):4249-60. doi: 10.1093/nar/4.12.4249.
9
Equilibrium studies of ethidium--polynucleotide interactions.溴化乙锭与多核苷酸相互作用的平衡研究。
Biochemistry. 1981 Jun 9;20(12):3547-53. doi: 10.1021/bi00515a038.
10
Uracil in deoxyribonucleotide polymers reduces their template-primer activity for E. coli DNA polymerase I.脱氧核糖核苷酸聚合物中的尿嘧啶会降低其作为大肠杆菌DNA聚合酶I模板引物的活性。
Nucleic Acids Res. 1983 Jun 11;11(11):3753-65. doi: 10.1093/nar/11.11.3753.
引用本文的文献
1
The Impact of Secondary Structure on the Base-Filling of N-Methoxy-1,3-Oxazinane (MOANA) and N-Methoxy-1,3-Oxazolidine Glycol Nucleic Acid (MOGNA) Oligonucleotides.二级结构对N-甲氧基-1,3-恶嗪烷（MOANA）和N-甲氧基-1,3-恶唑烷二醇核酸（MOGNA）寡核苷酸碱基填充的影响
Chembiochem. 2025 Jan 2;26(1):e202400666. doi: 10.1002/cbic.202400666. Epub 2024 Oct 27.
2
Real-time tracking reveals catalytic roles for the two DNA binding sites of Rad51.实时追踪揭示了 Rad51 两个 DNA 结合位点的催化作用。
Nat Commun. 2020 Jun 11;11(1):2950. doi: 10.1038/s41467-020-16750-3.
3
Homopolymer tail-mediated ligation PCR: a streamlined and highly efficient method for DNA cloning and library construction.均聚物尾巴介导的连接 PCR：一种用于 DNA 克隆和文库构建的简化、高效的方法。
Biotechniques. 2013 Jan;54(1):25-34. doi: 10.2144/000113981.
4
Fluorescence probing of T box antiterminator RNA: insights into riboswitch discernment of the tRNA discriminator base.T盒抗终止子RNA的荧光探测：对tRNA鉴别碱基核糖开关识别的见解。
Biochem Biophys Res Commun. 2009 Nov 27;389(4):616-21. doi: 10.1016/j.bbrc.2009.09.037. Epub 2009 Sep 13.
5
Thermodynamic studies of base pairing involving 2,6-diaminopurine.涉及2,6-二氨基嘌呤碱基配对的热力学研究。
Nucleic Acids Res. 1988 Jun 10;16(11):5115-22. doi: 10.1093/nar/16.11.5115.
本文引用的文献
1
A new polynucleotide complex stabilized by 3 interbase hydrogen bonds, poly-2-aminoadenylic acid + polyuridylic acid.一种由3个碱基间氢键稳定的新型多核苷酸复合物，聚-2-氨基腺苷酸 + 聚尿苷酸。
J Biol Chem. 1966 Sep 25;241(18):4293-5.
2
Comparative properties of DNA, RNA, and hybrid homopolymer pairs.DNA、RNA及杂交同聚物对的比较特性
Fed Proc. 1965 Nov-Dec;24(6):1446-57.
3
4
The interaction of actinomycin with DNA: requirement for the 2-amino group of purines.放线菌素与DNA的相互作用：嘌呤2-氨基基团的必要性。
Proc Natl Acad Sci U S A. 1967 Apr;57(4):1036-42. doi: 10.1073/pnas.57.4.1036.
5
The influence of neighboring base pairs upon base-pair substitution mutation rates.相邻碱基对对碱基对替换突变率的影响。
Proc Natl Acad Sci U S A. 1971 Apr;68(4):773-6. doi: 10.1073/pnas.68.4.773.
6
Studies on the pathway of incorporation of 2-aminopurine into the deoxyribonucleic acid of Escherichia coli.关于2-氨基嘌呤掺入大肠杆菌脱氧核糖核酸途径的研究。
J Bacteriol. 1970 Sep;103(3):622-33. doi: 10.1128/jb.103.3.622-633.1970.
7
Studies on the biochemical basis of spontaneous mutation. II. The incorporation of a base and its analogue into DNA by wild-type, mutator and antimutator DNA polymerases.自发突变的生化基础研究。II. 野生型、诱变型和抗诱变型DNA聚合酶将碱基及其类似物掺入DNA的过程。
J Mol Biol. 1974 Sep 15;88(2):409-21. doi: 10.1016/0022-2836(74)90491-4.
8
Preparation and properties of poly 2-aminopurine ribotidylic acid.聚2-氨基嘌呤核糖核苷酸的制备与性质
Acta Biochim Pol. 1973;20(3):271-84.
9
Enzyme kinetic parameters of the fluorescent ATP analogue, 2-aminopurine triphosphate.荧光ATP类似物2-氨基嘌呤三磷酸的酶动力学参数。
FEBS Lett. 1973 Jun 1;32(2):267-9. doi: 10.1016/0014-5793(73)80849-x.
10
The effects of thioketo substitution upon uracil-adenine interactions in polyribonucleotides. Synthesis and properties of poly (2-thiouridylic acid) and poly(2,4-dithiouridylic acid).硫酮取代对多聚核糖核苷酸中尿嘧啶 - 腺嘌呤相互作用的影响。聚（2 - 硫代尿苷酸）和聚（2,4 - 二硫代尿苷酸）的合成与性质
Eur J Biochem. 1973 Mar 15;33(3):535-44. doi: 10.1111/j.1432-1033.1973.tb02713.x.
Zotero 插件