多核苷酸。XL。聚2'-叠氮基-2'-脱氧腺苷酸的合成及性质
Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid.
作者信息
Ikehara M, Fukui T, Kakiuchi N
出版信息
Nucleic Acids Res. 1976 Aug;3(8):2089-99. doi: 10.1093/nar/3.8.2089.
Abstract
Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ellipticity. Titration of poly (Az) with HCl gave a transition at pH 5.5, but exact structure of the acid-form complex was not elucidated. Upon mixing with poly (U), poly (Az) forms a 1:1 and 1:2 complexes having Tm's somewhat higher than that of poly (A)- poly (U) complex in the same condition.
摘要
聚2'-叠氮基-2'-脱氧腺苷酸（聚叠氮酸）由2'-叠氮基-2'-脱氧二磷酸腺苷通过多核苷酸磷酸化酶合成。聚（叠氮酸）具有与聚（A）相似的紫外吸收特性，在0.1 M Na⁺和中性条件下有40%的减色效应。圆二色曲线也与聚（A）相似，但椭圆率较小。用HCl滴定聚（叠氮酸）在pH 5.5时出现转变，但酸形式复合物的确切结构尚未阐明。与聚（U）混合时，聚（叠氮酸）形成1:1和1:2的复合物，其熔解温度在相同条件下略高于聚（A）-聚（U）复合物。
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