使用多层酶联免疫吸附测定法检测粪便样本中溶组织内阿米巴滋养体抗原的初步观察。

Preliminary observations using a multi-layer ELISA method for the detection of Entamoeba histolytica trophozoite antigens in stool samples.

作者信息

Grundy M S

出版信息

Trans R Soc Trop Med Hyg. 1982;76(3):396-400. doi: 10.1016/0035-9203(82)90199-7.

Abstract

A method for detecting Entamoeba histolytica trophozoite antigens in aqueous solution is described. This involves coating plastic microtitre plates with a 'catching antibody', specific rabbit anti-E. histolytica antibody (SRAE). After adding the test material the presence of antigen is determined using two additional heterologous antibody layers, one of 'developing antibody', in this case human anti-E. histolytica immunoglobulin (HAE), which is followed by a final layer of peroxidase conjugated sheep anti-human immunoglobulin antibody (SH-HRP). The specificity and sensitivity of the assay was investigated both in the model system and using stool samples from infected patients. In the model system, the test had a sensitivity equivalent to detection of approximately one amoeba per microscope coverslip (18 mm X 18 mm). Little cross reaction was observed with other intestinal parasites common to the area of Bangladesh from which the stool samples were taken. The possible use of this method in large scale screening of stool samples and in the detection of circulating antigens is discussed.

摘要

本文描述了一种检测水溶液中溶组织内阿米巴滋养体抗原的方法。该方法包括用“捕获抗体”，即特异性兔抗溶组织内阿米巴抗体（SRAE）包被塑料微量滴定板。加入测试材料后，使用另外两层异源抗体来确定抗原的存在，一层是“显色抗体”，在这种情况下是人类抗溶组织内阿米巴免疫球蛋白（HAE），随后是最后一层过氧化物酶标记的羊抗人免疫球蛋白抗体（SH-HRP）。在模型系统和使用感染患者的粪便样本中研究了该检测方法的特异性和敏感性。在模型系统中，该测试的灵敏度相当于每块显微镜盖玻片（18 mm×18 mm）检测到约一个阿米巴。在所采集粪便样本的孟加拉国地区常见的其他肠道寄生虫中，未观察到明显的交叉反应。讨论了该方法在大规模筛查粪便样本和检测循环抗原中的可能用途。