Trechsel U, Dew G, Murphy G, Reynolds J J
Biochim Biophys Acta. 1982 Jul 22;720(4):364-70. doi: 10.1016/0167-4889(82)90113-6.
Collagenase secretion was studied on cultures of rabbit articular chondrocytes. Differentiation of the cells was assessed by characterizing the type of 3H-labelled collagen produced during treatment with (1) conditioned media from rabbit peritoneal macrophages and human blood mononuclear cells, and (2) with retinol, a potent cartilage resorbing agent in tissue culture. Conditioned media stimulated collagenase secretion. Total collagen synthesis was reduced due to a decrease of synthesis of alpha 1 chains; the amount of alpha 2 chains synthesized was unchanged. This is thought to be due to a reduction in type II synthesis. Retinol did not stimulate collagenase secretion. Total collagen synthesis was reduced by retinol. alpha 2 chain synthesis, however, was significantly increased, suggesting a switch of collagen synthesis in favor of type I collagen, and therefore, dedifferentiation. These results demonstrate that dedifferentiation of chondrocytes with respect to collagen synthesis is not necessarily associated with a stimulation of collagenase secretion.
在兔关节软骨细胞培养物上研究了胶原酶的分泌。通过鉴定在用以下物质处理期间产生的3H标记胶原的类型来评估细胞的分化：（1）兔腹膜巨噬细胞和人血单核细胞的条件培养基，以及（2）视黄醇，一种组织培养中有效的软骨吸收剂。条件培养基刺激胶原酶分泌。由于α1链合成减少，总胶原合成降低；合成的α2链数量未变。这被认为是由于II型合成减少所致。视黄醇不刺激胶原酶分泌。视黄醇使总胶原合成减少。然而，α2链合成显著增加，表明胶原合成转向有利于I型胶原，因此发生去分化。这些结果表明，软骨细胞在胶原合成方面的去分化不一定与胶原酶分泌的刺激相关。
