单纯疱疹病毒DNA克隆“连接区”的稳定性
Stability of the cloned 'joint region' of herpes simplex virus DNA.
作者信息
Russell R J, Kudler L, Miller R H, Hyman R W
出版信息
Intervirology. 1982;18(1-2):98-104. doi: 10.1159/000149312.
To isolate stable recombinants containing the 'joint region', or L-S junction, of herpes simplex virus DNA, the EcoRI restriction enzyme cleavage fragments were cloned into both coliphage lambda and plasmid vectors. The authentic joint region was found in the plasmid but not in the lambda vector. The plasmid-joint region recombinant DNAs appeared stable on limited passage. Subcloning the small BamHI L-S junction fragment into plasmid pBR322 gave rise to both stable and unstable recombinant DNAs.
为了分离含有单纯疱疹病毒DNA“连接区”或L-S连接点的稳定重组体，将EcoRI限制性内切酶切割片段克隆到大肠杆菌噬菌体λ和质粒载体中。在质粒中发现了真实的连接区，而在λ载体中未发现。质粒-连接区重组DNA在有限传代时显得稳定。将小的BamHI L-S连接片段亚克隆到质粒pBR322中产生了稳定和不稳定的重组DNA。
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