Colberg-Poley A M, Carter B J, Enquist L W
Gene. 1983 Oct;24(2-3):327-30. doi: 10.1016/0378-1119(83)90093-8.
Large fragments of Herpes simplex virus type 1 (HSV-1, strain KOS) DNA were produced by partial cleavage with Sau3A and inserted into a phage lambda BamHI vector. Recombinant phage (lambda KOS) DNA molecules were isolated and characterised. The final collection of phage recombinants contains partially overlapping inserts, which represent most of the HSV-1 genome. Restriction enzyme analysis of many independent clones containing Us sequences revealed sequence polymorphism in two specific regions.
通过用Sau3A进行部分酶切产生了1型单纯疱疹病毒（HSV-1，KOS株）的大片段DNA，并将其插入噬菌体λ BamHI载体中。分离并鉴定了重组噬菌体（λ KOS）DNA分子。最终收集的噬菌体重组体包含部分重叠的插入片段，这些片段代表了HSV-1基因组的大部分。对许多含有Us序列的独立克隆进行限制性酶切分析，发现在两个特定区域存在序列多态性。
