小鼠β-干扰素制剂对小鼠腹腔巨噬细胞吞噬作用及环磷酸腺苷水平的影响。

Effect of murine beta-interferon preparation on phagocytosis and cyclic AMP levels in mouse peritoneal macrophages.

作者信息

Degré M, Rollag H

出版信息

J Interferon Res. 1982;2(2):151-7. doi: 10.1089/jir.1982.2.151.

DOI:10.1089/jir.1982.2.151

Abstract

Mouse peritoneal macrophages (MPM) cultivated with a mouse beta-interferon preparation (MuIFN-beta) or "mock" IFN were tested for phagocytic ability and intracellular levels of adenosine 3',5'-cyclic monophosphate (cAMP). Suspensions of nonopsonized Escherichia coli (E. coli) were used for phagocytosis experiments. Treatment of MPM with 10(1)-10(3) U per ml of MuIFN-beta stimulated the phagocytic activity and raised the levels of cAMP in MPM. The effect of MuIFN-beta on cAMP levels were dose and time dependent. Maximal cAMP levels were seen when MPM were incubated with 10(3)-10(4) U per ml of MuIFN-beta for five hours. Simultaneous addition of MuIFN-beta and the adenylcyclase inhibitor N-ethylmaleimide to the MPM cultures prevented the rise in cAMP levels but not the increased phagocytic capacity. MuIFN-beta induced enhancement of phagocytosis and elevation of cAMP levels in MPM seem to be two parallel but not interlinked processes.

摘要

用小鼠β干扰素制剂（MuIFN-β）或“模拟”干扰素培养的小鼠腹腔巨噬细胞（MPM），检测其吞噬能力和细胞内3',5'-环磷酸腺苷（cAMP）水平。未调理的大肠杆菌（E. coli）悬液用于吞噬实验。用每毫升10(1)-10(3)单位的MuIFN-β处理MPM可刺激吞噬活性并提高MPM中的cAMP水平。MuIFN-β对cAMP水平的影响呈剂量和时间依赖性。当MPM与每毫升10(3)-10(4)单位的MuIFN-β孵育5小时时，可观察到最大cAMP水平。在MPM培养物中同时加入MuIFN-β和腺苷酸环化酶抑制剂N-乙基马来酰亚胺可阻止cAMP水平升高，但不能阻止吞噬能力增强。MuIFN-β诱导的MPM吞噬作用增强和cAMP水平升高似乎是两个平行但不相关的过程。