β-干扰素对小鼠腹腔巨噬细胞体外铺展的影响。

Effect of beta-interferon on in vitro spreading of mouse peritoneal macrophages.

作者信息

Rollag H, Degré M

出版信息

Acta Pathol Microbiol Immunol Scand B. 1982 Dec;90(6):389-95. doi: 10.1111/j.1699-0463.1982.tb00136.x.

DOI:10.1111/j.1699-0463.1982.tb00136.x

Abstract

The influence of a homologous beta-interferon preparation (MuIFN-beta) on in vitro adherence and the early and late phase of spreading of mouse peritoneal macrophages (MPM) was tested. MPM from mice pre-treated with 2 x 10(4) U of MuIFN-beta did not differ in ability to adhere to a glass surface, compared to controls. However, when the early phase of MPM spreading was tested, the percentage of spreading cells from mice injected with MuIFN-beta or Newcastle disease virus was almost doubled as compared to MPM from control mice. To assay the late phase of MPM spreading, MPM from unstimulated mice were seeded on glass surfaces and cultivated with or without MuIFN-beta for 1-24 hours. MuIFN-beta enhanced the spreading of MPM with maximal difference 3-5 hours after seeding. The effect was dose-dependent up to 10(2)U per ml of MuIFN-beta. The effect was also species--specific and neutralized by addition of anti-MuIFN-beta globulin. Morphometric measurements of the spreading of MPM indicated that after 24 hours of MuIFN-beta treatment the mean area occupied was 50 per cent larger than that of controls.

摘要

测试了一种同源β干扰素制剂（MuIFN-β）对小鼠腹腔巨噬细胞（MPM）体外黏附以及铺展早期和晚期的影响。与对照组相比，用2×10⁴U的MuIFN-β预处理的小鼠的MPM在黏附玻璃表面的能力上没有差异。然而，当测试MPM铺展的早期阶段时，与对照小鼠的MPM相比，注射MuIFN-β或新城疫病毒的小鼠的铺展细胞百分比几乎增加了一倍。为了测定MPM铺展的晚期阶段，将未受刺激小鼠的MPM接种在玻璃表面上，并在有或没有MuIFN-β的情况下培养1至24小时。MuIFN-β增强了MPM的铺展，接种后3至5小时差异最大。该效应在每毫升MuIFN-β高达10²U时呈剂量依赖性。该效应也是物种特异性的，并可通过添加抗MuIFN-β球蛋白来中和。MPM铺展的形态测量表明，MuIFN-β处理24小时后，平均占据面积比对照组大50%。