使用鲎凝血酶的合成显色底物对人血进行高氯酸处理以进行内毒素定量测定。
Perchloric acid treatment of human blood for quantitative endotoxin assay using synthetic chromogenic substrate for horseshoe crab clotting enzyme.
作者信息
Tamura H, Obayashi T, Takagi K, Tanaka S, Nakahara C, Kawai T
出版信息
Thromb Res. 1982 Jul 1;27(1):51-7. doi: 10.1016/0049-3848(82)90277-8.
A new reliable human blood treatment was established for quantitative endotoxin assay using synthetic chromogenic substrate [Boc-Leu-Gly-Arg-rho-nitroanilide]. Addition of perchloric acid in a final concentration of 1.25% to platelet-rich plasma or serum in a 2:1 volume ratio completely eliminated nonspecific amidase activities as well as inhibitors. By this method, the recovery of added endotoxin was nearly 100%, and was almost independent of sample dilutions and anticoagulants.
建立了一种新的可靠的人体血液处理方法，用于使用合成显色底物[Boc-Leu-Gly-Arg-ρ-硝基苯胺]进行内毒素定量测定。以2:1的体积比向富含血小板的血浆或血清中加入终浓度为1.25%的高氯酸，可完全消除非特异性酰胺酶活性以及抑制剂。通过这种方法，添加内毒素的回收率接近100%，并且几乎与样品稀释度和抗凝剂无关。
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